Transport activities involved in intracellular pH recovery following acid and alkali challenges in rat brain microvascular endothelial cells.

Pflügers Archiv : European journal of physiology
Pieris A NicolaS B Hladky

Abstract

Transport activities involved in intracellular pH (pH(i)) recovery after acid or alkali challenge were investigated in cultured rat brain microvascular endothelial cells by monitoring pH(i) using a pH-sensitive dye. Following relatively small acid loads with pH(i) approximately 6.5, HCO(-)(3) influx accounted for most of the acid extrusion from the cell with both Cl(-)-independent and Cl(-)-dependent, Na(+)-dependent transporters involved. The Cl(-)-independent component has the same properties as the NBC-like transporter previously shown to account for most of the acid extrusion near the resting pH(i). Following large acid loads with pH(i) < 6.5, most of the acid extrusion was mediated by Na(+)/H(+) exchange, the rate of which was steeply dependent on pH(i). Concanamycin A, an inhibitor of V-type ATPase, had no effect on the rates of acid extrusion. Following an alkali challenge, the major component of the acid loading leading to recovery of pH(i) occurred by Cl(-)/HCO(-)(3) exchange. This exchange had the same properties as the AE-like transporter previously identified as a major acid loader near resting pH(i). These acid-loading and acid-extruding transport mechanisms together with the Na(+), K(+), ATPase may be sufficient t...Continue Reading

References

Oct 1, 1992·The American Journal of Physiology·D BrownI Sabolić
Dec 1, 1991·The American Journal of Physiology·J D Clark, L E Limbird
Jan 1, 1990·The American Journal of Physiology·P Y LinE Gruenstein
Jan 1, 1986·Annual Review of Cell Biology·Q Al-Awqati
Jan 1, 1985·Annual Review of Physiology·P S Aronson
Mar 1, 1985·The Journal of General Physiology·L Simchowitz, A Roos
Mar 1, 1986·The Journal of Cell Biology·S OlsnesK Sandvig
Dec 1, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R D Vaughan-Jones
Oct 1, 1983·Journal of Neurochemistry·A L Betz
Apr 1, 1981·Physiological Reviews·A Roos, W F Boron
Jun 1, 1994·Journal of Cellular Physiology·J C SeagraveJ R Martinez
Oct 1, 1994·The American Journal of Physiology·H M Maldonado, P M Cala
Sep 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Y Ou-yangB K Siesjö
Nov 1, 1995·The Journal of General Physiology·E M HoganW F Boron
Jul 1, 1996·Proceedings of the Society for Experimental Biology and Medicine·P HsuC W Leffler
May 12, 1998·The Journal of Physiology·C H Leem, R D Vaughan-Jones
Aug 7, 1998·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·S FaberE Mutschler
Jul 17, 1999·The American Journal of Physiology·B SunJ Kambayashi
Sep 8, 2001·American Journal of Physiology. Cell Physiology·A K StewartS L Alper
May 9, 2002·American Journal of Physiology. Cell Physiology·D GrangerR Laprade
Jun 21, 2005·Cellular and Molecular Neurobiology·Hdikó SiposVera Adam-Vizi
Aug 19, 2006·The Journal of Physiology·Caroline J TaylorStephen B Hladky

❮ Previous
Next ❯

Citations

Sep 12, 2013·Pflügers Archiv : European journal of physiology·Ruth MokgokongStephen B Hladky
Jan 23, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Masaoki TakanoShogo Matsuyama
May 22, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Pascal AndréSalvatore Cisternino
Jan 9, 2014·The Journal of International Medical Research·Chi-Yu XuCui-Jie Liu
Jun 8, 2018·Molecular Pharmaceutics·Huilong LuoXavier Declèves

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Blood-Brain Barrier Transport in Neurodegeneration

The blood brain barrier is important for regulating the movement of biomolecules in and out of the brain. For example, membrane transporters in the blood brain barrier can be essential for regulating drug movement and dysregulation of these processes may play a role in neurodegeneration. This feed follows the latest research on this topic.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.