PMID: 9546035Apr 18, 1998Paper

Transport and endogenous release of vitamin B12 in the dually perfused human placenta

The Journal of Pediatrics
R E Perez-D'Gregorio, R K Miller

Abstract

The kinetics for vitamin B12, cobalamin (Cbl), transfer across the human placenta, and the retention of the endogenous Cbl and its release into the maternal and fetal compartments were investigated in a dually perfused human term placenta in vitro. After 4 hours of perfusion following a single bolus injection (peak maternal perfusate 94 fmol/ml) of 57Co-Cbl into the maternal reservoir, the maternal [57Co-Cbl] rapidly decreased whereas the fetal [57Co-Cbl] was only 9% of the final maternal [57Co-Cbl]. Even though there was a limited transfer of 57Co-Cbl to the fetus, the placenta rapidly accumulated Cbl. At 4 hours, 18% of the initial 57Co-Cbl dose was in the placenta; only 3% of the initial dose was in the fetal perfusate. Also after 4 hours, [57Co-Cbl] was bound > 95% to transcobalamin (TC)I/III-like proteins in the maternal perfusate, whereas Cbl was bound to TCI/III- and TCII-like proteins with some free in the fetal perfusate. In the cytosol, > 95% of the [57Co-Cbl] was bound (80% to TCII-like and 19% to TCI/III-like proteins). When no exogenous Cbl was added, total endogenous [Cbl] in the maternal circulation increased with time during 8 hours of perfusion at a rate of 0.25 +/- 0.12 pmol/gm per hour. Only 2% of this Cbl wa...Continue Reading

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Citations

Jun 6, 2000·Advanced Drug Delivery Reviews·G A ChandorkarK L Audus
Dec 7, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anupa R MenjogeRangaramanujam M Kannan
Jul 26, 2016·Frontiers in Molecular Biosciences·Luciana HannibalHenk J Blom
Mar 26, 2003·QJM : Monthly Journal of the Association of Physicians·J G Ray, H J Blom
May 10, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Luciana HannibalDonald W Jacobsen
Apr 21, 2010·Journal of Pediatric Hematology/oncology·Siddharth BankaWilliam G Newman
Jan 16, 2004·The Journal of Physiology·Seema KalraBellur Seetharam
Jun 21, 2003·The American Journal of Clinical Nutrition·Aśok C Antony
Sep 17, 2015·Advances in Nutrition·Julia L FinkelsteinPatrick J Stover
Sep 1, 2016·Placenta·Alexander J LaydenJulia L Finkelstein
Dec 16, 2021·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Pooja DhimanSoundravally Rajendiran

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