Transport and metabolism of the essential vitamin pantothenic acid in human erythrocytes infected with the malaria parasite Plasmodium falciparum.

The Journal of Biological Chemistry
Kevin J SalibaKiaran Kirk

Abstract

The growth of the human malaria parasite, Plasmodium falciparum, within its host erythrocyte is reliant on the uptake of a number of essential nutrients from the extracellular medium. One of these is pantothenic acid, a water-soluble vitamin that is a precursor of coenzyme A. In this study we show that normal uninfected erythrocytes are impermeable to pantothenate but that the vitamin is taken up rapidly into malaria-infected cells via a transport pathway that has the characteristics (furosemide sensitivity, nonsaturability) of previously characterized, broad specificity permeation pathways induced by the intracellular parasite in the host cell membrane. The transport of pantothenate therefore constitutes a critical physiological role for these pathways. Inside the parasitized cell pantothenate undergoes phosphorylation, the first step in its conversion to coenzyme A. Parasites within saponin-permeabilized erythrocytes were shown to take up and phosphorylate pantothenate, consistent with the intracellular parasite having both a pantothenate transporter and a pantothenate kinase. Comparisons of the rate of phosphorylation of pantothenate by lysates prepared from uninfected and infected erythrocytes revealed that the pantothenate...Continue Reading

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·F H Brohn, W Trager
Feb 1, 1978·Annals of Tropical Medicine and Parasitology·G PasvolJ Brown
Jan 1, 1991·Vitamins and Hormones·A G Tahiliani, C J Beinlich
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B A BiggsG V Brown
Jun 14, 1995·Biochimica Et Biophysica Acta·J M Upston, A M Gero
May 1, 1997·Transactions of the Royal Society of Tropical Medicine and Hygiene·S L CranmerB M Cooke

❮ Previous
Next ❯

Citations

Nov 19, 2010·The Journal of Membrane Biology·Mayra Delgado-RamírezOxana R Dobrovinskaya
May 24, 2005·Pflügers Archiv : European journal of physiology·Christophe DurantonFlorian Lang
Jul 28, 2010·Medical & Biological Engineering & Computing·Jakob M A MauritzClemens F Kaminski
May 4, 2004·Blood Cells, Molecules & Diseases·Virgilio L LewTeresa Tiffert
Oct 18, 2003·Molecular and Biochemical Parasitology·Jamieson V CohnSanjay A Desai
Oct 18, 2003·Molecular and Biochemical Parasitology·Stefan BaumeisterKlaus Lingelbach
Jan 1, 2004·Trends in Parasitology·Richard J W Allen, Kiaran Kirk
Sep 22, 2001·International Journal for Parasitology·S KrishnaC Woodrow
Sep 22, 2001·International Journal for Parasitology·K J Saliba, K Kirk
Jan 26, 2002·The International Journal of Biochemistry & Cell Biology·Xiaohua NiYumin Mao
Apr 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Peter EllekvistNirbhay Kumar
Jul 9, 2009·The Journal of Biological Chemistry·Christina Spry, Kevin J Saliba
Mar 31, 2004·The Journal of General Physiology·Christophe DurantonFlorian Lang
Nov 1, 1995·British Journal of Haematology·J A WeidanzA C Vezza
Jul 25, 2003·Antimicrobial Agents and Chemotherapy·Giancarlo A BiaginiStephen A Ward
Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Kevin J SalibaKiaran Kirk
Sep 5, 2006·Eukaryotic Cell·Godfrey LiskSanjay A Desai
Apr 4, 2009·PLoS Computational Biology·Jakob M A MauritzVirgilio L Lew
Mar 29, 2013·PloS One·Donelly A van SchalkwykKiaran Kirk
Apr 22, 2014·PloS One·Jessica K O'HaraManuel Llinás
Jul 25, 2013·Blood Cells, Molecules & Diseases·Surendra K Prajapati, Om P Singh
Sep 23, 2014·BioMed Research International·Abdullah A B BokhariSanjay A Desai
Sep 3, 2014·Biomedical Journal·Ghania Ramdani, Gordon Langsley
Jan 23, 2016·Nature Communications·Sanketha KenthirapalanTaco W A Kooij
Nov 8, 2006·Cellular Microbiology·Abdulnaser AlkhalilSanjay A Desai
Jan 16, 2008·Molecular Microbiology·Cecilia P SanchezMichael Lanzer
Dec 12, 2013·The Biochemical Journal·Kiaran Kirk, Adele M Lehane
Sep 7, 2012·The Biochemical Journal·Margery A BarrandStephen B Hladky

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.