PMID: 2599Feb 25, 1976

Transport and metabolism of vitamin B6 in Salmonella typhimurium LT2.

The Journal of Biological Chemistry
J H Mulligan, E E Snell


Salmonella typhimurium LT2 concentrates radioactivity intracellularly from [3H]pyridoxal or [3H]pyridoxine up to 25 times the external concentration. After 1 min of uptake intracellular radioactivity is found as phosphorylated vitamin B6. The process is sensitive to temperature and is maximally active at pH 8.1, but under the conditions tested it is insensitive to monovalent cations or metabolic inhibitors, and does not require an exogenous energy source. The Km values for uptake of pyridoxine and pyridoxal are 2.0 x 10(-7) M and 1.2 x 10(-7) M, respectively; [3H]pyridoxamine is not transported. Evidence is presented for an uptake mechanism involving facilitated diffusion followed by trapping by pyridoxal kinase. S. typhimurium also appears to lack a periplasmic binding protein for vitamin B6.

Related Concepts

Biological Transport, Active
Hydrogen-Ion Concentration
Osmotic Stress
Rodex brand of pyridoxine hydrochloride
Salmonella typhimurium LT2

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

The Journal of Biological Chemistry
J H Mulligan, E E Snell
Journal of Nutritional Science and Vitaminology
T SakuraiM Matsuda
Investigative Ophthalmology & Visual Science
J J DuttonB Becker
Archives of Biochemistry and Biophysics
A Kozik, D B McCormick
The Journal of Nutrition
B B Bowman, D B McCormick
© 2021 Meta ULC. All rights reserved