Transport and signaling through the phosphate-binding site of the yeast Pho84 phosphate transceptor.

Proceedings of the National Academy of Sciences of the United States of America
Yulia PopovaJohan M Thevelein

Abstract

A novel concept in eukaryotic signal transduction is the use of nutrient transporters and closely related proteins as nutrient sensors. The action mechanism of these "transceptors" is unclear. The Pho84 phosphate transceptor in yeast transports phosphate and mediates rapid phosphate activation of the protein kinase A (PKA) pathway during growth induction. We have now identified several phosphate-containing compounds that act as nontransported signaling agonists of Pho84. This indicates that signaling does not require complete transport of the substrate. For the nontransported agonist glycerol-3-phosphate (Gly3P), we show that it is transported by two other carriers, Git1 and Pho91, without triggering signaling. Gly3P is a competitive inhibitor of transport through Pho84, indicating direct interaction with its phosphate-binding site. We also identified phosphonoacetic acid as a competitive inhibitor of transport without agonist function for signaling. This indicates that binding of a compound into the phosphate-binding site of Pho84 is not enough to trigger signaling. Apparently, signaling requires a specific conformational change that may be part of, but does not require, the complete transport cycle. Using Substituted Cysteine...Continue Reading

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Citations

Oct 1, 2011·Molecular Genetics and Genomics : MGG·Sandra C Dos Santos, Isabel Sá-Correia
Apr 2, 2013·Nature·Bjørn P PedersenRobert M Stroud
Sep 8, 2010·The Journal of Biological Chemistry·Christine SalaünGérard Friedlander
Jan 18, 2011·Journal of Experimental Botany·Alain GojonEdith Laugier
Feb 14, 2013·Journal of Experimental Botany·Oliver BerkowitzHans Lambers
Dec 27, 2011·Plant & Cell Physiology·Vinay K Nagarajan, Aaron P Smith
Mar 16, 2012·Genetics·Per O Ljungdahl, Bertrand Daignan-Fornier
Mar 22, 2014·Progress in Biophysics and Molecular Biology·Gábor Maksay, Orsolya Tőke
May 23, 2014·Journal of Biosciences·Parul Tomar, Himanshu Sinha
Oct 12, 2013·Current Genetics·Joep SchothorstJohan M Thevelein
Sep 18, 2010·Cellular and Molecular Life Sciences : CMLS·Solmaz KhoshniatLaurent Beck
Jun 21, 2011·Trends in Plant Science·Benjamin PéretThierry Desnos
May 17, 2011·Current Opinion in Plant Biology·Steffen Abel
Jul 9, 2014·Trends in Plant Science·Tzu-Yin LiuTzyy-Jen Chiou
Jun 22, 2010·Journal of Cellular Biochemistry·Kaisa KarhumaaMorten C Kielland-Brandt
Jan 15, 2014·Journal of Integrative Plant Biology·Zhaoliang ZhangWilliam J Lucas
May 23, 2012·Cellular & Molecular Biology Letters·Fernando CardonaAgustín Aranda
Dec 17, 2014·Biotechnology and Bioengineering·Jinrui ZhangSebastian Aljoscha Wahl
Jun 19, 2013·Biochemical and Biophysical Research Communications·Palanivelu SengottaiyanBengt L Persson
Dec 15, 2010·The Biochemical Journal·Ruben GhillebertJoris Winderickx
Feb 4, 2014·FEMS Microbiology Reviews·Michaela ConradJohan M Thevelein
Jun 1, 2014·Experimental Cell Research·Iben Boutrup KongsfeltLene Pedersen
Aug 31, 2016·Frontiers in Plant Science·Ewa Młodzińska, Magdalena Zboińska
Apr 9, 2014·Infection and Immunity·Matthias KretschmerJames W Kronstad

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