Transport and utilization of amino acids and glucose in human monocytes: activation of glucose metabolism by insulin

The Journal of Clinical Endocrinology and Metabolism
W BiegerJ Michl

Abstract

The influence of insulin on transport and utilization of amino acids and glucose in purified human peripheral blood monocytes has been studied. Insulin had an immediate stimulating effect on the uptake of 3-O-methylglucose and 2-deoxyglucose; the maximal effects were 55% and 47% increases, respectively, during the first 2 min, in which energy-dependent hexose uptake dominates. Later, with advancing free diffusion, values declined to 16% and 25%. After a lag of 30 min, the rise in glucose uptake was followed by a small rise in glucose oxidation, documented by an 18% increase of 14CO2 production from [1-14C]glucose in the presence of hormone. No effect of insulin on sodium dependent alpha-aminoisobutyric acid or sodium-independent leucine uptake in monocytes could be found. The incorporation of amino acids into monocyte protein remained unchanged as well. Our results prove that the well documented binding of insulin to human monocytes initiates specific cellular reactions. The increased hexose monophosphate shunt activity may result in increased immune reactivity of the monocyte.

Citations

Jul 20, 2006·Journal of Medical Systems·Em Ward, Terry Martin
Mar 1, 1987·Molecular and Cellular Endocrinology·A P BautistaA Volkman
Jun 15, 2007·Current Opinion in Clinical Nutrition and Metabolic Care·Philip C CalderPhilip Newsholme
Jul 21, 2010·European Journal of Endocrinology·Eirini MaratouGeorge Dimitriadis
Jan 15, 2009·European Journal of Endocrinology·Eirini MaratouGeorge Dimitriadis
Mar 17, 2009·Diabetes Research and Clinical Practice·Yorgi MavrosMaria A Fiatarone Singh
Feb 3, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·George DimitriadisSotirios A Raptis
Mar 22, 2007·European Journal of Clinical Investigation·E MaratouS A Raptis
Feb 1, 1982·The American Journal of Physiology·J P Filkins, M R Yelich
Mar 17, 2021·Journal of Leukocyte Biology·Walter David Cruz-PinedaOlga Lilia Garibay-Cerdenares
Dec 1, 1994·The American Journal of Physiology·Z SpolaricsJ J Spitzer
Jul 15, 1992·Biochemical and Biophysical Research Communications·Z SpolaricsJ J Spitzer

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