May 28, 1976

Transport and utilization of the biosynthetic intermediate shikimic acid in Escherichia coli

Biochimica Et Biophysica Acta
K D Brown, C H Doy

Abstract

Auxotrophic mutants of Escherichia coli W or K12 blocked before shikimic acid in the aromatic biosynthetic pathway grew poorly on shikimic acid as sole aromatic supplement. This poor growth response was correlated with a relatively poor ability to transport shikimic acid. If citrate was present in the growth medium (as it is in some commonly used basal media) the growth of some of the E. coli K12 mutants on shikimate was further reduced. Mutants were derived from pre-shikimate auxotrophs which grew rapidly on media containing shikimic acid. These derivatives all had an increased ability to transport shikimic acid. Thus, it is proposed that the growth on shikimate observed in the parent cells is restricted by their relatively poor uptake of shikimate from the medium and that this restriction may be removed by a mutation which enhances shikimate transport. Transduction analysis of the mutations which enhanced utilization and transport of shikimic acid by E. coli K12 strains indicated at least two classes. Class 1 was about 20% cotransduced with the histidine region of the E. coli K12 chromosome and appeared to be coincident with a known shikimate transport locus, shiA. Class 2 was not cotransduced with his. The locus (or loci) of...Continue Reading

  • References5
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References

Mentioned in this Paper

Shikimic Acid
Derivatives
Alkalescens-Dispar Group
Histidine
Uptake
Recombination, Genetic
Transduction
Shikimate Transport
Citrate Measurement
Cell Division Phases

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