Transport-associated phosphorylation of 2-deoxy-D-glucose in Saccharomyces fragilis.

Biochimica Et Biophysica Acta
H T Jaspers, J van Steveninck

Abstract

2-Deoxy-D-glucose transport and metabolism was studied in Saccharomyces fragilis. Inside the cells four phosphorylated and three non-phosphorylated derivatives were found and identified. Accumulation of phosphorylated 2-deoxyglucose derivatives was balanced by a concomitant decrease of cellular ATP, orthophosphate and polyphosphates. The free sugar was concentrated against a concentration gradient, contradiciting facilitated diffusion. Pulse labeling experiments revealed transport-associated phosphorylation. Theoretical considerations and analysis of the effects of iodoacetate showed that an intracellular hexokinase activity was not involved in 2-deoxyglucose phosphorylation, although this sugar is a good substrate for the enzyme in in vitro experiments.

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Citations

Jul 7, 1977·Molecular & General Genetics : MGG·K W van de Poll, D H Schamhart
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