PMID: 2492158Jan 1, 1989Paper

Transport characteristics of glutamine in human intestinal brush-border membrane vesicles

The American Journal of Physiology
H M SaidR Redha

Abstract

Transport of glutamine (Gln) across the brush-border membrane of the human intestine was examined using the brush-border membrane vesicle (BBMV) technique. Osmolarity and temperature studies indicate that the uptake of Gln by BBMV is mostly the result of transport of the substrate into the intravesicular space. Transport of Gln was Na+ gradient dependent (out greater than in) with a distinct "overshoot" phenomenon. Initial rate of transport of Gln as a function of concentration was saturable both in the presence and absence of a Na+ gradient (out greater than in). Apparent Km of 1.86 and 1.36 mM and Vmax of 1,906 and 637 pmol.mg protein-1 . 7 s-1 were calculated for the Na+-dependent and the Na+-independent transport processes of Gln, respectively. The transport of [3H]Gln (0.58 mM) by the Na+-dependent and the Na+-independent processes was severely inhibited by the addition to the incubation medium of serine, asparagine, and unlabeled Gln. Inducing a relatively negative intravesicular compartment with the use of valinomycin and an outwardly directed K+ gradient or with the use of anions of different lipid permeabilities indicates that Gln transport by the Na+-dependent process is electrogenic in nature. Transport of Gln by the...Continue Reading

Citations

Jan 22, 2005·Journal of Applied Physiology·Moïse CoëffierPierre Déchelotte

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