PMID: 6106503Oct 2, 1980Paper

Transport characteristics of papain-treated brush-border membrane vesicles. Non-involvement of gamma-glutamyltransferase in leucine transport

Biochimica Et Biophysica Acta
A BertelootK Ramaswamy

Abstract

Papain treatment of isolated brush border membrane vesicles was carried out to correlate directly the solubilization of gamma-glutamyltransferase with the uptake of leucine. Digestion of membrane vesicles with either soluble or gel-complexed papain resulted in nearly complete removal of gamma-glutamyltransferase. However, the treated vesicles exhibited increased specific activity of leucine and glucose uptake, indicating the non-involvement of the transferase in leucine transport. The partial purification of amino acid and sugar transport function was better controlled with gel-complexed papain. In contrast to the digestion with soluble papain, the treatment with gel-complexed papain did not modify the diffusional components for solutes and ions and did not alter the intravesicular volume. It appears that controlled papain-digestion, resulting in nearly a 2-fold purification of the transport function with high reproducibility and quantitative recovery of uptake, should be useful in future attempts to purify the 'carrier' proteins.

References

Jan 1, 1976·Annual Review of Biochemistry·A Meister, S S Tate
Mar 20, 1978·Biochimica Et Biophysica Acta·R HahnL Flohé
Jul 3, 1978·European Journal of Biochemistry·S HoriuchiY Morino
Jan 1, 1977·Reviews of Physiology, Biochemistry and Pharmacology·R K Crane
Apr 1, 1975·Archives of Biochemistry and Biophysics·T Kuhlenschmidt, N P Curthoys
Oct 17, 1975·Biochimica Et Biophysica Acta·H SigristG Semenza
Jan 12, 1976·Biochemical and Biophysical Research Communications·K RamaswamyR K Crane
May 21, 1976·Biochimica Et Biophysica Acta·D Maestracci
Apr 6, 1973·Science·A Meister
Sep 27, 1973·Biochimica Et Biophysica Acta·J SchmitzR K Crane
May 5, 1973·Biochimica Et Biophysica Acta·D LouvardP Desnuelle
Oct 1, 1970·Physiological Reviews·S G Schultz, P F Curran
Nov 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·L NaftalinD Tracey
Sep 1, 1969·Analytical Biochemistry·J B Lloyd, W J Whelan
Jan 1, 1972·FEBS Letters·H Glossmann, D M. Neville
Apr 15, 1973·FEBS Letters·M OrlowskiJ P. Green
Jan 1, 1964·Analytical Biochemistry·A DAHLQVIST

❮ Previous
Next ❯

Citations

Nov 30, 1982·Biochimica Et Biophysica Acta·I Nemere, A W Norman
Apr 6, 1981·Biochimica Et Biophysica Acta·V GanapathyF H Leibach
Dec 19, 1984·Biochimica Et Biophysica Acta·V M RajendranK Ramaswamy
Jan 1, 1984·Comparative Biochemistry and Physiology. A, Comparative Physiology·S LaganièreD Maestracci
Jun 6, 1989·Biochimica Et Biophysica Acta·K RamaswamyJ A Barry
Nov 17, 1989·Biochimica Et Biophysica Acta·D WilsonK Ramaswamy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.