Abstract
Nicotine is absorbed from the lungs into the systemic circulation during cigarette smoking. However, there is little information concerning the transport mechanism of nicotine in alveolar epithelial cells. In this study, we characterized the uptake of nicotine in rat primary cultured type II (TII) and transdifferentiated type I-like (TIL) epithelial cells. In both TIL and TII cells, [(3)H]nicotine uptake was time and temperature-dependent, and showed saturation kinetics. [(3)H]Nicotine uptake in these cells was not affected by Na(+), but was sensitive to extracellular and intracellular pH, suggesting the involvement of a nicotine/proton antiport system. The uptake of [(3)H]nicotine in these cells was potently inhibited by organic cations such as clonidine, diphenhydramine, and pyrilamine, but was not affected by substrates and/or inhibitors of known organic cation transporters such as carnitine, 1-methyl-4-phenylpyridinium, and tetraethylammonium. In addition, the uptake of [(3)H]nicotine in TIL cells was stimulated by preloading the cells with unlabeled nicotine, pyrilamine, and diphenhydramine, but not with tetraethylammonium. These results suggest that a novel proton-coupled antiporter is involved in the uptake of nicotine i...Continue Reading
References
Feb 1, 1975·Experimental and Molecular Pathology·M J EvansG Freeman
Jul 27, 1992·Biochimica Et Biophysica Acta·M TakanoR Hori
Feb 18, 1987·Biochimica Et Biophysica Acta·A W JansR K Kinne
Dec 1, 1988·Journal of Cellular Physiology·T D McKinneyK V Speeg
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D W NielsonJ A Clements
Jun 11, 1998·Journal of Pharmaceutical Sciences·S ZevinK M Giacomini
Jul 21, 1999·Journal of the National Cancer Institute·S S Hecht
Nov 1, 2001·Respiratory Research·H Fehrenbach
Jul 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Atsuko FukadaKen-Ichi Inui
Nov 13, 2002·Annual Review of Physiology·Mary C Williams
Dec 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Lee CampbellMark Gumbleton
Sep 28, 2004·The Journal of Biological Chemistry·Karen EngelJoanne Wang
Mar 3, 2005·Lung·Amelia W NgThomas A Heming
Dec 14, 2006·Cell and Tissue Research·Sibylle EndterCarsten Ehrhardt
Sep 14, 2007·Pharmaceutical Research·Mika IkehataMikihisa Takano
Jul 9, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Takashi OkuraYoshiharu Deguchi
Sep 17, 2008·Gastroenterology Research and Practice·J R IngramG A O Thomas
Jan 1, 2009·Arthritis and Rheumatism·Marjolein A van MaanenPaul P Tak
Feb 3, 2009·Handbook of Experimental Pharmacology·Neal L BenowitzPeyton Jacob
May 2, 2009·The Journal of Pharmacy and Pharmacology·Sibylle EndterMark Gumbleton
Oct 6, 2009·Biological & Pharmaceutical Bulletin·Mika IkehataMikihisa Takano
Apr 13, 2010·Pulmonary Pharmacology & Therapeutics·John D Taylor
Sep 4, 2010·Drug Metabolism and Pharmacokinetics·Mikihisa TakanoRyoko Yumoto
Jul 15, 2011·Pharmacological Reviews·Anders S KristensenUlrik Gether
Dec 6, 2012·The AAPS Journal·Salvatore CisterninoJean-Michel Scherrmann
Dec 12, 2012·Neurochemistry International·Yuma TegaKen-Ichi Hosoya
Aug 31, 2013·Life Sciences·Mikihisa TakanoRyoko Yumoto
Dec 17, 2014·Expert Opinion on Drug Delivery·Mikihisa TakanoRyoko Yumoto
Apr 22, 2015·Journal of Pharmaceutical Sciences·Yuma TegaKen-Ichi Hosoya
Citations
Nov 12, 2016·Drug Metabolism and Pharmacokinetics·Mikihisa TakanoRyoko Yumoto
Nov 14, 2016·Human Cell·Shigeki OritaniTakaki Ishikawa
Apr 8, 2020·Journal of Analytical Methods in Chemistry·Vinit V GholapMatthew S Halquist
Sep 4, 2018·Biological & Pharmaceutical Bulletin·Yuma TegaKen-Ichi Hosoya
Aug 28, 2020·Expert Opinion on Drug Delivery·Vinit V GholapMatthew S Halquist
Sep 4, 2017·Life Sciences·Mikihisa TakanoRyoko Yumoto
May 6, 2021·Respiratory Research·Yanxia WangZhichao Li