PMID: 9528682Apr 7, 1998Paper

Transport mechanisms for vitamin C in the JAR human placental choriocarcinoma cell line

Biochimica Et Biophysica Acta
P D PrasadV Ganapathy

Abstract

We investigated the transport pathways available for the uptake of vitamin C in the human placental choriocarcinoma cell line, JAR. These cells were found to possess the capacity to accumulate the vitamin when presented either in the oxidized form (dehydroascorbic acid) or in the reduced form (ascorbate). Dithiothreitol and 5,5'-dithiobis(2-nitrobenzoic acid) were used to maintain vitamin C as ascorbate and dehydroascorbic acid, respectively. The uptake of these two forms of vitamin C in JAR cells was found to occur by different mechanisms. The uptake of the dehydroascorbic acid was Na(+)-independent and was mediated by facilitative glucose transporters as evidenced from the inhibition of the uptake process by glucose. On the other hand, the uptake of ascorbate was Na(+)-dependent and was not sensitive to inhibition by glucose. Substitution of Na+ with other monovalent cations abolished the uptake of ascorbate completely. The uptake process was, however, not influenced by anions. Kinetic analysis indicated the presence of a single saturable transport system for ascorbate with a Michaelis-Menten constant of 22 +/- 1 microM. The dependence of the uptake rare of ascorbate on Na+ concentration exhibited sigmoidal kinetics, suggesti...Continue Reading

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Citations

May 29, 2008·Pflügers Archiv : European journal of physiology·Maite A CastroIlona I Concha
Feb 21, 2002·The Biochemical Journal·Melanie E HolmesAshok K Grover
Jun 18, 2002·European Journal of Clinical Investigation·G PéterszegiL Robert
Jul 20, 1999·European Journal of Biochemistry·H LaggnerH Goldenberg
Jun 9, 2001·Molecular Membrane Biology·W J LiangS M Jarvis
Jan 13, 2000·Biochemical and Biophysical Research Communications·Y WangM A Hediger

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