PMID: 1201274Dec 16, 1975Paper

Transport of 8-anilino-1-naphthalenesulfonate as a probe of the effect of cholesterol on the phospholipid bilayer structures

Biochemistry
T Y Tson-

Abstract

The transport of 8-anilino-1-naphthalenesulfonate in dimyristoyl-L-alpha-lecithin bilayers has been found to be extremely sensitive to the crystalline state of the phospholipid dispersions. Thus this reaction may be used for probing the membrane structures. In binary mixtures of cholesterol and phospholipid the fluorescence enhancement of the dye completely disappears when the mole fraction of cholesterol reaches 33%. At temperatures below and above the phase transition of the lipid bilayers, the rate of the probe transport increases significantly in the binary mixtures. It reaches a maximum at 17 mol % of cholestero. The rate at this cholesterol content approaches the maximum value obtained for the probe transport in pure phospholipis, e.i., the rate at the midpoint of the phase transition. These observations indicate that the effect of cholesterol in the phospholipid dispersion is to maintain the bilayer structure close to the melting temperature of the lipid phase transition. In other words, cholesterol may be an effective buffer for membrane crystalline state when its concentration is near 17 mol %.

Citations

Mar 8, 1977·The Journal of Membrane Biology·E F LaBelle, E Racker
Jan 29, 1986·Biochimica Et Biophysica Acta·W K Subczynski, A Kusumi
Feb 27, 1989·Biochimica Et Biophysica Acta·L Cruzeiro-HanssonO G Mouritsen
Nov 22, 1982·Biochimica Et Biophysica Acta·R M EpandS W Hui
Jul 12, 1977·Biochemistry·T Y TsongM I Kanehisa
Jun 14, 1977·Biochemistry·T Y Tsong, M I Kanehisa
Jun 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Grażyna NeunertKrzysztof Polewski
Aug 11, 1982·Biochimica Et Biophysica Acta·J Slavík

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