Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm.

Applied and Environmental Microbiology
J T TrevorsM E Starodub

Abstract

Vertical soil microcosms flushed with groundwater were used to study the influence of water movement on survival and transport of a genetically engineered Pseudomonas fluorescens C5t strain through a loamy sand and a loam soil. Transport of cells introduced into the top 1 cm of the vertical soil microcosms was dependent on the flow rate of water and the number of times microcosms were flushed with groundwater. The presence of wheat roots growing downward in the microcosms contributed only slightly to the movement of P. fluorescens C5t cells to lower soil regions of the loamy sand microcosms, but enhanced downward transport in the loam microcosms. Furthermore, the introduced P. fluorescens C5t cells were detected in the effluent water samples even after three flushes of groundwater and 10 days of incubation. As evidenced by a comparison of counts from immunofluorescence and selective plating, nonculturable C5t cells occurred in day 10 soil and percolated water samples, primarily of the loamy sand microcosms. Vertical soil microcosms that use water movement may be useful in studying the survival and transport of genetically engineered bacteria in soil under a variety of conditions prior to field testing.

References

Nov 1, 1986·Applied and Environmental Microbiology·H FestlK H Schleifer
Oct 1, 1988·Applied and Environmental Microbiology·G CompeauS B Levy
Nov 1, 1988·Applied and Environmental Microbiology·K R Gurijala, M Alexander
Sep 1, 1988·Applied and Environmental Microbiology·R J Steffan, R M Atlas
Jul 1, 1986·Canadian Journal of Microbiology·J T Trevors, K M Oddie
Jun 1, 1968·Australian Journal of Biological Sciences·D M Griffin, G Quail
Mar 1, 1977·Applied and Environmental Microbiology·S A Schaub, C A Sorber
Nov 1, 1983·Applied and Environmental Microbiology·S Soby, K Bergman
Mar 1, 1988·Applied and Environmental Microbiology·William E HolbenJames M Tiedje
May 1, 1989·Applied and Environmental Microbiology·P G HartelJ W Williamson

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Citations

Jun 1, 1991·Applied Microbiology and Biotechnology·J T Trevors
Sep 3, 2005·Nature Reviews. Microbiology·Søren J SørensenStefan Wuertz
Oct 24, 2008·Phytopathology·W E SnyderD A Kluepfel
Mar 18, 2015·FEMS Microbiology Ecology·Alexandra B WolfGeorge A Kowalchuk
Oct 31, 2000·Applied and Environmental Microbiology·W E Holben, P H Ostrom
Aug 6, 2004·Applied and Environmental Microbiology·Oliver G G KnoxMichael Wilson
May 10, 2006·Ground Water·Hailiang DongMark E Fuller
Dec 16, 2003·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·M Katherine BanksRao S Govindaraju
Apr 5, 2013·FEMS Microbiology Reviews·Michiel VosGeorge A Kowalchuk
Jan 1, 1995·Antonie van Leeuwenhoek·J T Trevors
May 7, 2004·Applied and Environmental Microbiology·Fulvio CelicoGino Naclerio
Jan 1, 1994·The Journal of Applied Bacteriology·A Ramos-CormenzanaL F Gomez-Palma
Sep 3, 2003·FEMS Microbiology Letters·O G G KnoxM J Wilson
Mar 1, 1995·Applied Soil Ecology : a Section of Agriculture, Ecosystems & Environment·Jane L MawdsleyMichael K Theodorou
Oct 1, 1993·Applied and Environmental Microbiology·H H RijnaartsA J Zehnder
Jun 1, 1993·Applied and Environmental Microbiology·C A Bellin, P S Rao
Jun 1, 1994·Applied and Environmental Microbiology·Y Bashan, G Holguin
Mar 16, 2004·Water Research·Tor Kristian StevikJon Fredrik Hanssen

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