PMID: 3758220Aug 1, 1986Paper

Transport of acidic amino acids by the bovine pigment epithelium

Experimental Eye Research
E L Pautler, C Tengerdy

Abstract

The regulation of acidic amino-acid transport across the retinal pigment epithelium is of particular interest since glutamate and possibly aspartate have been identified as putative neurotransmitters in the retina, at the level of the photoreceptor cell. The present study, designed to measure the rate of acidic amino-acid transport across the mammalian pigment epithelium (PE), shows that there is a net transport of both glutamate and aspartate in the retina to choroid direction (R-C), with the R-C unidirectional flux of glutamate being substantially larger than the corresponding aspartate flux. The R-C and C-R fluxes of glutamate were found to be inhibited by ouabain. Further investigations utilizing aspartate revealed that the fluxes in both directions were inhibited when ouabain was present on the retinal side of the tissue preparation. The R-C flux of glutamate was significantly reduced by lowered concentrations of Na+, K+ and Ca2+, whereas the C-R flux was diminished only by the reduced concentration Ca2+. The changes in K+ concentration which markedly altered the R-C flux of glutamate were within the range of light-induced changes of K+ which has been observed in the extracellular space of the photoreceptor cells. The tran...Continue Reading

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Oct 1, 1982·Experimental Eye Research·C E Crosson, E L Pautler

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Citations

Feb 1, 1993·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·R Salceda, M R Saldaña
Oct 26, 1999·Progress in Retinal and Eye Research·M Kalloniatis, G Tomisich
May 1, 1990·Photochemistry and Photobiology·E L PautlerD Beezley
Jan 10, 2002·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Ali A HussainJohn Marshall
Aug 1, 1987·Experimental Eye Research·E L Pautler, F L Hall
Jan 1, 1991·Drug Metabolism Reviews·F J Leinweber
Nov 13, 2002·Pharmacology & Toxicology·Hanna MäenpääHanna Tähti
Dec 16, 1996·The Journal of Comparative Neurology·E L Fletcher, M Kalloniatis
May 1, 1999·Alternatives to Laboratory Animals : ATLA·H TähtiT Toimela
Nov 1, 1989·The American Journal of Physiology·E L PautlerD Beezley
Nov 24, 2018·Experimental Eye Research·Nina Buus SørensenJens Folke Kiilgaard

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