PMID: 7033209Jan 1, 1982

Transport of alpha-aminoisobutyric acid by Streptococcus pyogenes and its derived L-form

Journal of Bacteriology
J Reizer, C Panos

Abstract

We studied the uptake of alpha-aminoisobutyric acid (AIB) in Streptococcus pyogenes and its physiologically isotonic L-form. S. pyogenes cells starved for glucose or treated with carbonyl cyanide-m-chlorophenyl hydrazone accumulated limited amounts of AIB. A high apparent K(m) value characterized the glucose-independent transport of AIB. The rate and extent of AIB accumulation significantly increased in the presence of glucose. Two saturable transport components with distinct apparent K(m) values characterized glycolysis-coupled transport of AIB. A biphasic Lineweaver-Burk plot was also obtained for l-alanine transport by glycolyzing S. pyogenes cells. AIB seems to share a common transport system(s) with glycine, l- and d-alanine, l-serine, and l-valine. This was shown by the competitive inhibition of AIB uptake by these compounds and their ability to induce competitive exchange efflux of accumulated AIB. About 30% of the AIB uptake was not inhibited by a saturating amount of l-valine, indicating the existence of more than one system for AIB transport. p-Chloromercuribenzoate markedly inhibited the accumulation of AIB by both glycolyzing and glucose-starved cells. In contrast, carbonyl cyanide-m-chlorophenyl hydrazone affected ...Continue Reading

Citations

Feb 14, 2009·The Journal of Antimicrobial Chemotherapy·Pearl M SweRalph W Jack
Sep 1, 1993·FEMS Microbiology Reviews·B Poolman
Dec 1, 1985·Journal of Applied Toxicology : JAT·J ScatinaE Goldman
Jan 1, 1989·Critical Reviews in Microbiology·W N KoningsA J Driessen
Aug 9, 2006·Critical Reviews in Microbiology·María Fernández, Manuel Zúñiga
Feb 15, 2011·Journal of Bacteriology·Breah LaSarre, Michael J Federle

Related Concepts

Abufne
Aminoisobutyric Acids
Cations, Divalent
Cations, Monovalent
Glycolysis
Hydrogen-Ion Concentration
L Forms
Bacteria, Flesh-Eating
Substrate Specificity

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