PMID: 2492498Jan 1, 1989Paper

Transport of amino acids in Lactobacillus casei by proton-motive-force-dependent and non-proton-motive-force-dependent mechanisms

Journal of Bacteriology
H J StrobelW N Konings

Abstract

Lactobacillus casei 393 cells which were energized with glucose (pH 6.0) took up glutamine, asparagine, glutamate, aspartate, leucine, and phenylalanine. Little or no uptake of several essential amino acids (valine, isoleucine, arginine, cysteine, tyrosine, and tryptophan) was observed. Inhibition studies indicated that there were at least five amino acid carriers, for glutamine, asparagine, glutamate/aspartate, phenylalanine, or branched-chain amino acids. Transport activities had pH optima between 5.5 and 6.0, but all amino acid carriers showed significant activity even at pH 4.0. Leucine and phenylalanine transport decreased markedly when the pH was increased to 7.5. Inhibitors which decreased proton motive force (delta p) nearly eliminated leucine and phenylalanine uptake, and studies with de-energized cells and membrane vesicles showed that an artificial electrical potential (delta psi) of at least -100 mV was needed for rapid uptake. An artificial delta p was unable to drive glutamine, asparagine, or glutamate uptake, and transport of these amino acids was sensitive to a decline in intracellular pH. When intracellular pH was greater than 7.7, glutamine, asparagine, or glutamate was transported rapidly even though the prot...Continue Reading

References

Aug 1, 1979·Journal of Bacteriology·G B HendersonF M Huennekens
Nov 1, 1987·Journal of Bacteriology·A J DriessenW N Konings
Dec 1, 1987·Microbiological Reviews·B PoolmanW N Konings
Jan 1, 1986·Annual Review of Biochemistry·G F Ames
Oct 1, 1980·Journal of Bacteriology·S K Rhee, M Y Pack

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Citations

Sep 1, 1993·FEMS Microbiology Reviews·B Poolman
Jun 1, 1993·Oral Microbiology and Immunology·S G Dashper, E C Reynolds
Dec 27, 1990·European Journal of Biochemistry·R Krämer, C Lambert
Jun 1, 1995·Oral Microbiology and Immunology·S G DashperE C Reynolds
May 1, 1994·World Journal of Microbiology & Biotechnology·G S de Giori, G F de Valdez
Jul 31, 2002·Journal of Applied Microbiology·F M Saguir, M C Manca de Nadra
Aug 9, 2006·Critical Reviews in Microbiology·María Fernández, Manuel Zúñiga
Oct 1, 1990·Journal of Dairy Science·J B RussellS A Martin
Sep 28, 2007·Journal of Applied Microbiology·N VermeulenR F Vogel
Dec 11, 1991·Antimicrobial Agents and Chemotherapy·N E AllenJ N Hobbs
Dec 1, 1991·Applied and Environmental Microbiology·M J Starrenburg, J Hugenholtz
Nov 11, 2008·Cell Host & Microbe·Dirk HofreuterJorge E Galán

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