PMID: 11912047Mar 26, 2002Paper

Transport of cadmium across the apical membrane of epithelial cell lines

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Tetsuya Endo

Abstract

Cadmium (Cd) uptake and secretion across the apical membrane of epithelial cells was studied using LLC-PK1 cells cultured on Petri dishes and permeable membranes, respectively. Cd accumulation in cells from the apical medium was decreased by low temperature and metabolic inhibitors. A saturable tendency was observed between initial Cd accumulation and increased concentrations of Cd in the apical medium at 37 degrees C, but not at 4 degrees C. Co-incubation with ZnCl2 or CuCl2 competitively decreased Cd accumulation at 37 degrees C. A decrease in the pH of the apical medium markedly decreased Cd accumulation. Pretreatment of cells with an inorganic anion-exchange inhibitor significantly decreased Cd uptake at pH 7.4 in the presence of bicarbonate, but only marginally in its absence. A decrease in the pH of the apical medium increased the secretory (basolateral-to-apical) transport of Cd, with a concomitant decrease in the cellular accumulation of Cd. Co-incubation with Cd and tetraethylammonium, a typical substrate of the organic cation transporter, decreased Cd transport, with a concomitant increase in cellular Cd accumulation. The uptake and secretion of Cd across the apical membrane appear to be partly mediated via an inorgan...Continue Reading

References

Dec 31, 1991·Biochemical and Biophysical Research Communications·W C Prozialeck, R J Niewenhuis
Nov 1, 1991·The Journal of Physiology·M LouJ O Alda
Feb 1, 1990·The Journal of Physiology·V Kalfakakou, T J Simons
Feb 1, 1984·Journal of Cellular Physiology·F V Sepúlveda, J D Pearson
Oct 1, 1993·Physiological Reviews·J B Pritchard, D S Miller
Apr 1, 1996·Toxicology and Applied Pharmacology·O KimuraM Sakata
Dec 1, 1995·Biological & Pharmaceutical Bulletin·T EndoM Sakata
Jul 1, 1996·Biological & Pharmaceutical Bulletin·T EndoM Sakata
Feb 1, 1997·Toxicology and Applied Pharmacology·E A PigmanH E Laird
Mar 24, 1999·Current Opinion in Hematology·N C AndrewsJ E Levy
Jul 8, 1999·Journal of Cellular Physiology·C JumarieF Denizeau

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Citations

Mar 7, 2003·Toxicology and Applied Pharmacology·Rudolfs K Zalups, Sarfaraz Ahmad
Apr 2, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Takuya KawanaiYasuo Oyama
Oct 21, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M VázquezV Devesa
Oct 31, 2007·Experimental Eye Research·N K WillsF J G M van Kuijk
Apr 23, 2005·Toxicology and Applied Pharmacology·Christy C Bridges, Rudolfs K Zalups
Nov 14, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Francesca TrinchellaRosaria Scudiero
Apr 22, 2011·Metallomics : Integrated Biometal Science·Hitomi FujishiroSeiichiro Himeno
Sep 15, 2005·Journal of the American Chemical Society·Corey R CenterwallJames C Dabrowiak

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