PMID: 4722898Jan 1, 1973Paper

Transport of glutamine and glutamate in kidney mitochondria in relation to glutamine deamidation

The Biochemical Journal
M Crompton, J B Chappell


1. In the absence of added ADP glutamine is transformed by pig kidney mitochondria to ammonium glutamate, which appears in the external medium. This reaction is stimulated only slightly by the addition of ADP, but under these conditions about 20% of the glutamate is oxidized to aspartate. 2. Externally added glutamate is oxidized to aspartate, and at about the same rate as glutamine. 3. The net rates of glutamine and glutamate influx into the intramitochondrial compartment are very slow. 4. The phosphate-dependent glutaminase activity of intact mitochondria is stimulated by the provision of energy. 5. The provision of energy also decreases the concentration of glutamate and increases the concentration of glutamine in the intramitochondrial compartment. These energy-linked changes in the glutamine and glutamate concentrations are of equal magnitude. 6. It is suggested that transport of glutamine and glutamate across the inner membrane of kidney mitochondria occurs by an obligatory exchange between the two metabolites, and is electrogenic. The existence of an electrogenic glutamine-glutamate anti-porter is proposed.


Feb 1, 1982·Kidney International·R S SleeperH G Preuss
Mar 15, 1975·FEBS Letters·J D McGivan, J B Chappell
Jan 1, 1982·Acta neurochirurgica·L Symon, S Mohanty
Apr 23, 1976·Molecular & General Genetics : MGG·A Sainsard-Chanet
Nov 15, 1975·FEBS Letters·S M Danks, J B Chappel
Jul 1, 1980·The American Journal of Physiology·T C Welbourne, G T Bazer
Dec 1, 1981·The American Journal of Physiology·R N Heitmann, E N Bergman

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