Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging

Optics Letters
Shan Shan KouColin J Sheppard

Abstract

Differential interference contrast (DIC) microscopy is an inherently qualitative phase-imaging technique. What is obtained is an image with mixed phase-gradient and amplitude information rather than a true linear mapping of actual optical path length (OPL) differences. Here we investigate an approach that combines the transport-of-intensity equation (TIE) with DIC microscopy, thus improving direct visual observation. There is little hardware modification and the computation is noniterative. Numerically solving for the propagation of light in a series of through-focus DIC images allows linear phase information in a single slice to be completely determined and restored from DIC intensity values.

References

Mar 9, 2000·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·C Preza
Oct 10, 2002·Applied Optics·Colin J R Sheppard
Mar 31, 2004·Journal of Microscopy·M R ArnisonC J Cogswell
Nov 24, 2004·Ultramicroscopy·M BeleggiaY Zhu
Feb 9, 2006·Applied Optics·Michael Shribak, Shinya Inoué
Dec 19, 2007·Optics Letters·A BartyA Roberts
Mar 10, 1997·Applied Optics·D S Biggs, M Andrews

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Citations

May 15, 2013·Annual Review of Biomedical Engineering·P MarquetP J Magistretti
Mar 9, 2013·Zygote : the Biology of Gametes and Early Embryos·G CoppolaB Dale
Nov 26, 2014·Nature Methods·Thomas A Zangle, Michael A Teitell
Jul 11, 2011·IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society·Serhan O IsikmanAydogan Ozcan
Dec 20, 2015·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Emrah BostanMichael Unser
Apr 18, 2014·Biophysical Journal·Pierre BonSandrine Lévêque-Fort
Apr 26, 2017·Optics Express·Tonmoy Chakraborty, Jonathan C Petruccelli
Mar 1, 2018·Journal of Biomedical Optics·Aihui SunCheng Liu
Mar 11, 2017·Journal of Biomedical Optics·Yudong YaoJianQiang Zhu
Sep 1, 2017·Journal of Biomedical Optics·Jing XuShouyu Wang
Mar 9, 2010·Journal of Biophotonics·Bertrand SimonOlivier Haeberlé
Sep 19, 2017·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Kevin P BohannonDaniel Axelrod
Dec 18, 2010·Optics Express·Laura WallerGeorge Barbastathis
Oct 17, 2014·Applied Optics·JaeHwang JungYongKeun Park
Dec 4, 2012·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Scott A PrahlDonald D Duncan
May 4, 2016·Optics Express·YoonSeok BaekYongKeun Park
Sep 26, 2019·Scientific Reports·Congli WangWolfgang Heidrich
Feb 23, 2011·Biomedical Optics Express·Lisa MiccioPietro Ferraro
Mar 4, 2011·Optics Express·Laura WallerGeorge Barbastathis
Oct 28, 2015·Biomedical Optics Express·Jonghee YoonYongKeun Park
Mar 4, 2020·Optics Express·Congli WangWolfgang Heidrich
Sep 3, 2010·Optics Letters·Laura WallerGeorge Barbastathis
Jul 19, 2011·Optics Letters·Shan Shan KouColin J R Sheppard
Jun 7, 2011·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Donald D DuncanScott A Prahl
Nov 13, 2015·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Roman BarankovJerome Mertz
Jul 2, 2016·Optics Letters·Gennady VishnyakovValery Pickalov
Jul 19, 2017·Biomedical Optics Express·Aihui SunCheng Liu
Oct 3, 2012·Optics Letters·Ashwin B ParthasarathyJerome Mertz
Sep 21, 2011·Optics Letters·Ignacio Iglesias

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