PMID: 6408606Apr 1, 1983Paper

Transport of L-lysine by rat intestinal brush border membrane vesicles

Pflügers Archiv : European journal of physiology
G CassanoH Murer

Abstract

Brush border membranes were isolated from rat jejunum by a divalent cation precipitation method. 3H-L-Lysine uptake was measured by a rapid filtration technique. Uptake after prolonged incubation periods was osmotically insensitive and represented almost exclusively binding to the vesicles. Extrapolating initial linear uptake to a zero incubation time indicated no binding of the amino acid to the external membrane surface. Sodium did not significantly alter the initial uptake rate. L-Lysine transport represents a carrier mediated uptake in the presence and absence of sodium as indicated by the transstimulation experiments. The transport mechanism operates stereospecifically and is inhibited by other basic amino acids and L-leucine and L-phenylalanine. Saturation experiments result in a Km of 0.26 mmoles/l and a Vmax of 272 pmoles/mg protein/10s. Inside negative anion diffusion potentials and inside negative potassium diffusion potentials (valinomycin) were unable to increase the transport rate. Transmembrane pH-gradients were also unable to alter transport.

References

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Citations

Jan 30, 1989·Biochimica Et Biophysica Acta·C P Wheeler, D L Yudilevich
Jan 1, 1984·Comparative Biochemistry and Physiology. A, Comparative Physiology·S WolframE Scharrer
Jan 1, 1997·European Journal of Obstetrics, Gynecology, and Reproductive Biology·N CarbóJ M Argilés
Jul 30, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T HatanakaH Ushio
Mar 23, 2000·European Journal of Biochemistry·S BerardiS Krähenbühl
Jan 16, 2008·Physiological Reviews·Stefan Bröer
Mar 1, 1988·The Journal of Pharmacy and Pharmacology·H SaitohT Arita
Jan 1, 1991·Progress in Biophysics and Molecular Biology·C I Cheeseman
Sep 1, 1994·The American Journal of Physiology·M S MalandroD A Novak

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