PMID: 2492433Jan 30, 1989Paper

Transport of L-lysine in the fission yeast Schizosaccharomyces pombe

Biochimica Et Biophysica Acta
H SychrováA Kotyk

Abstract

Systems of L-lysine transport in Schizosaccharomyces pombe are not constitutive, as at no phase of growth in a rich medium is lysine taken up. Transport activity appears only after preincubation of harvested cells with glucose or another suitable source of energy. If cycloheximide is added during this preincubation no transport systems are synthesized. After removal of glucose, the activity of the transport system decays with a half-time of 13 min. The transport of L-lysine into S. pombe cells from the stationary phase of growth preincubated for 60 min with 1% D-glucose is mediated by at least two systems, the high-affinity one with a Kt of 26 mumol/l and Jmax of 4.95 nmol/min per mg dry wt., the low-affinity one with a KT of 1.1 mmol/l and Jmax of 11.8 nmol/min per mg dry wt. The transport of lysine mediated by these two systems proceeds uphill. The high-affinity system has a pH optimum at 4.0-4.2, the accumulation ratio is highest at a cell density 2-5 mg dry wt. per ml and decreases with increasing lysine concentrations. Lysine accumulated by this system does not exit from cells. The only potent competitive inhibitors are L-arginine, L-histidine and D-lysine. The other amino acids tested do not behave as competitive inhibito...Continue Reading

References

Feb 1, 1979·Journal of General Microbiology·A Kotyk, D Michaljanicová
May 1, 1977·Journal of Bacteriology·J R Woodward, V P Cirillo
Feb 1, 1978·Journal of Bacteriology·J M Beckerich, H Heslot
Jul 3, 1975·Biochimica Et Biophysica Acta·E H RamosA O Stoppani
Dec 22, 1986·Biochimica Et Biophysica Acta·J Horák
Apr 14, 1986·Biochimica Et Biophysica Acta·L R RaoR Prasad
Jan 1, 1985·Folia Microbiologica·S Janda, A Kotyk
Nov 2, 1972·Biochimica Et Biophysica Acta·A Kotyk, L Ríhová
Apr 1, 1983·Molecular and Cellular Biology·W E Courchesne, B Magasanik
Sep 27, 1982·Biochimica Et Biophysica Acta·A KotykA Knotková

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Citations

Jan 26, 2008·Molecular Genetics and Genomics : MGG·Paul-Joseph Aspuria, Fuyuhiko Tamanoi
Mar 22, 2002·The Journal of Eukaryotic Microbiology·M Basselin-EiweidaE S Kaneshiro
Apr 5, 2001·Biochimica Et Biophysica Acta·M BasselinE S Kaneshiro
Oct 1, 1993·FEMS Microbiology Letters·H SychrováA Kotyk
Nov 28, 2001·Molecular Pharmacology·K TsukaharaT Nagasu
Jun 21, 2001·Archives of Biochemistry and Biophysics·M BasselinE S Kaneshiro
Mar 14, 1997·Biochimica Et Biophysica Acta·J Horák

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