Transport of lucifer yellow CH into plant vacuoles--evidence for direct energization of a sulphonated substance and implications for the design of new molecular probes

FEBS Letters
M KleinGottfried Weissenböck

Abstract

Contrasting observations exist which indicate that in plants the fluorescent dye lucifer yellow CH (LYCH) either can be used as a tracer for endocytosis or as a substrate for an anion transporter located at the vacuolar membrane. In addition, LYCH as a disulphonated substance may represent an analogue of sulphonated or sulfated natural compounds like some flavonoids. We performed uptake experiments with LYCH into isolated rye vacuoles and observed saturable (Km = 0.3-0.6 mM) vacuolar transport and accumulation of the dye against the concentration gradient only when MgATP was present. GTP and, to a low extent, UTP could substitute for ATP, while the non-hydrolysable ATP analogue AMP-PNP did not drive LYCH uptake. Vanadate and probenecid, the latter substance is known to inhibit organic anion transport at the liver canalicular membrane, both strongly decreased the vacuolar uptake of LYCH, while bafilomycin A1, a specific inhibitor of the vacuolar H+-ATPase, had no effect. Together with the fact that abolishment of the delta pH via CCCP had only a weak influence on LYCH accumulation, our results indicate that this compound is taken up into rye vacuoles by a directly energized process. Uptake of LYCH was strongly inhibited by other...Continue Reading

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Citations

Nov 28, 2002·Pharmacology & Therapeutics·Bent H Havsteen
Nov 15, 2002·Fungal Genetics and Biology : FG & B·Helen A AtkinsonNick D Read
Oct 19, 2005·Protoplasma·F Aniento, D G Robinson
Nov 28, 2000·Plant & Cell Physiology·E MartinoiaN Frangne
Mar 16, 2012·The Plant Journal : for Cell and Molecular Biology·Wolfram AdlassnigIrene K Lichtscheidl
Oct 12, 1999·The American Journal of Physiology·N BallatoriJ L Boyer
Apr 5, 2000·Biochimica Et Biophysica Acta·F L Theodoulou

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