Transport of nucleosides across the Plasmodium falciparum parasite plasma membrane has characteristics of PfENT1

Molecular Microbiology
Megan J DownieKiaran Kirk

Abstract

Like all parasitic protozoa, the human malaria parasite Plasmodium falciparum lacks the enzymes required for de novo synthesis of purines and it is therefore reliant upon the salvage of these compounds from the external environment. P. falciparum equilibrative nucleoside transporter 1 (PfENT1) is a nucleoside transporter that has been localized to the plasma membrane of the intraerythrocytic form of the parasite. In this study we have characterized the transport of purine and pyrimidine nucleosides across the plasma membrane of 'isolated' trophozoite-stage P. falciparum parasites and compared the transport characteristics of the parasite with those of PfENT1 expressed in Xenopus oocytes. The transport of nucleosides into the parasite: (i) was, in the case of adenosine, inosine and thymidine, very fast, equilibrating within a few seconds; (ii) was of low affinity [K(m) (adenosine) = 1.45 +/- 0.25 mM; K(m) (thymidine) = 1.11 +/- 0.09 mM]; and (iii) showed 'cross-competition' for adenosine, inosine and thymidine, but not cytidine. The kinetic characteristics of nucleoside transport in intact parasites matched very closely those of PfENT1 expressed in Xenopus oocytes [K(m) (adenosine) = 1.86 +/- 0.28 mM; K(m) (thymidine) = 1.33 +/-...Continue Reading

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Citations

Sep 19, 2008·The Journal of Biological Chemistry·María B CasseraVern L Schramm
Oct 30, 2008·The Journal of Biological Chemistry·Dennis C MadridKami Kim
Jun 24, 2008·Eukaryotic Cell·Megan J DownieChoukri Ben Mamoun
Feb 11, 2016·International Journal for Parasitology. Drugs and Drug Resistance·Roman DeniskinMyles H Akabas
Aug 1, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J D YoungS A Baldwin
Apr 16, 2010·Trends in Parasitology·Henry M StainesSanjeev Krishna
May 9, 2009·Trends in Parasitology·Kiaran KirkMegan J Downie
Jun 19, 2007·Trends in Parasitology·Arnaud Ghérardi, Marie-Elisabeth Sarciron
Oct 3, 2009·Molecular Microbiology·Rowena E MartinKiaran Kirk
Nov 27, 2014·Annals of the New York Academy of Sciences·I J FrameMyles H Akabas
Dec 12, 2013·The Biochemical Journal·Kiaran Kirk, Adele M Lehane
Mar 8, 2020·Medicinal Research Reviews·Gustavo D Campagnaro, Harry P de Koning
May 5, 2010·The Journal of Biological Chemistry·Megan J DownieChoukri Ben Mamoun
Apr 11, 2018·Frontiers in Chemistry·Anna MeierEric Beitz

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