Transport of organic anions and cations in murine embryonic kidney development and in serially-reaggregated engineered kidneys

Scientific Reports
Melanie L LawrenceJamie A Davies

Abstract

Recent advances in renal tissue engineering have shown that dissociated, early renogenic tissue from the developing embryo can self-assemble into morphologically accurate kidney-like organs arranged around a central collecting duct tree. In order for such self-assembled kidneys to be useful therapeutically or as models for drug screening, it is necessary to demonstrate that they are functional. One of the main functional characteristics of mature kidneys is transport of organic anions and cations into and out of the proximal tubule. Here, we show that the transport function of embryonic kidneys allowed to develop in culture follows a developmental time-course that is comparable to embryonic kidney development in vivo. We also demonstrate that serially-reaggregated engineered kidneys can transport organic anions and cations through specific uptake and efflux channels. These results support the physiological relevance of kidneys grown in culture, a commonly used model for kidney development and research, and suggest that serially-reaggregated kidneys self-assembled from separated cells have some functional characteristics of intact kidneys.

References

Mar 8, 1995·Biochemical and Biophysical Research Communications·V GekelerJ Beck
Jul 1, 1997·DNA and Cell Biology·V GorboulevH Koepsell
Apr 6, 2000·American Journal of Physiology. Renal Physiology·A PavlovaS K Nigam
Sep 21, 2000·American Journal of Physiology. Renal Physiology·U KarbachH Koepsell
Jan 17, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·A L SlittC D Klaassen
May 23, 2002·The Journal of Pharmacology and Experimental Therapeutics·Atsushi EnomotoHitoshi Endou
Jul 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Phillip M Gerk, Mary Vore
Dec 25, 2002·European Journal of Pharmacology·Simon LowesNicholas L Simmons
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tsuyoshi MikkaichiTakaaki Abe
Apr 8, 2004·American Journal of Physiology. Renal Physiology·Geri L Youngblood, Douglas H Sweet
Jul 23, 2004·Physiological Reviews·Stephen H Wright, William H Dantzler
Mar 24, 2005·Pharmaceutical Research·Naoko KimuraKen-ichi Inui
Aug 5, 2005·The Journal of Pharmacology and Experimental Therapeutics·Naohiko AnzaiHitoshi Endou
Dec 31, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yazen AlnoutiCurtis D Klaassen
Jan 13, 2006·American Journal of Physiology. Renal Physiology·Takashi SekineHitoshi Endou
Dec 19, 2006·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Lene JørgensenLassina Badolo
Dec 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·E RosinesS K Nigam
Dec 18, 2009·Kidney International·Mathieu Unbekandt, Jamie A Davies
Jan 30, 2010·The American Journal of Pathology·Giuliano CiarimboliEberhard Schlatter
Aug 21, 2010·Journal of Pharmacological Sciences·Jeff Hughes, Andrew Crowe
Mar 26, 2011·Journal of Pharmaceutical Sciences·Tomoya YasujimaHiroaki Yuasa
Apr 16, 2011·Molecular Pharmacology·Derina E SweeneySanjay K Nigam
Oct 22, 2011·British Journal of Pharmacology·Megan RothBruno Hagenbuch
Mar 13, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·J William HigginsMaciej J Zamek-Gliszczynski
Oct 23, 2012·Journal of the American Society of Nephrology : JASN·Christodoulos XinarisGiuseppe Remuzzi
Oct 23, 2012·Organogenesis·Aleksandra Rak-RaszewskaPatricia Murray

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Citations

Jul 13, 2016·European Journal of Pharmacology·Tom T G Nieskens, Martijn J Wilmer
Jun 1, 2016·Scientific Reports·J JansenR Masereeuw
Jun 26, 2018·Expert Opinion on Biological Therapy·Francesca BecherucciPaola Romagnani
Aug 24, 2018·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Pedro Caetano-Pinto, Simone H Stahl
May 23, 2019·Médecine sciences : M/S·Clara SteichenThierry Hauet
May 11, 2018·Pflügers Archiv : European journal of physiology·Dorina Diekjürgen, David W Grainger
Sep 7, 2018·Biomaterials·Balajikarthick SubramanianJing Zhou

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Methods Mentioned

BETA
dissection
confocal microscopy
PCR

Software Mentioned

elements
Axiovision
NIS

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