PMID: 38845Sep 4, 1979

Transport of p-aminohippuric acid, uric acid and glucose in highly purified rabbit renal brush border membranes

Biochimica Et Biophysica Acta
I KippenE M Wright

Abstract

A procedure for preparing highly purified brush border membranes from rabbit kidney cortex using differential and density gradient centrifugation is described. Brush border membranes prepared by this procedure were substantially free of basal-lateral membranes, mitochondria, endoplasmic reticulum and nuclear material as evidenced by an enrichment factor of less than 0.3 for (Na+ + K+)-ATPase, succinate dehydrogenase, NADPH-cytochrome c reductase and DNA. Alkaline phosphatase was enriched ten fold indicating that the membranes were enriched at least 30 fold with respect to other cellular organelles. The yield of brush border membranes was 20%. Transport of D-glucose by the membranes was identical to that previously reported except that the Arrhenius plot for temperature dependence of transport was curvilinear (EA = 11.3--37.6 kcal/mol) rather than biphasic. Transport of p-aminohippuric acid and uric acid were increased by the presence of NaCl, either gradient or preequilibrated. However, no overshoot was obtained in the presence of a NaCl gradient, and KCl and LiCl also produced equivalent stimulation of transport suggesting a nonspecific ionic strength effect. Uptakes of p-aminohippuric acid and uric acid were not saturable, an...Continue Reading

References

May 1, 1993·Pflügers Archiv : European journal of physiology·C Schmitt, G Burckhardt
Jan 1, 1982·The Journal of Membrane Biology·A K MircheffE M Wright
Sep 1, 1996·British Journal of Pharmacology·R MasereeuwF G Russel
May 28, 1986·Biochimica Et Biophysica Acta·B Carrière, C Le Grimellec
Aug 1, 1986·Kidney International·H Murer, P Gmaj
Oct 1, 1992·Kidney International·C Le GrimellecM C Giocondi

Related Concepts

Centrifugation, Density Gradient
Tissue Membrane
Microvilli
Adenosine Triphosphatases
Succinate Dehydrogenase
Urate
Stimulation of Transport
Uptake
Alkaline Phosphatase Measurement
Glucose, (beta-D)-Isomer

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