PMID: 11607080Jun 1, 1990Paper

Transport of phosphocholine in higher plant cells: 31P nuclear magnetic resonance studies

Proceedings of the National Academy of Sciences of the United States of America
E GoutR Douce

Abstract

Phosphocholine (PC) is an abundant primary form of organic phosphate that is transported in plant xylem sap. Addition of PC to the perfusate of compressed Pi-starved sycamore cells monitored by 31P NMR spectroscopy resulted in an accumulation of PC and all the other phosphate esters in the cytoplasmic compartment. Addition of hemicholinium-3, an inhibitor of choline uptake, to the perfusate inhibited PC accumulation but not inorganic phosphate (Pi). When the Pi-starved cells were perfused with a medium containing either Pi or PC, the resulting Pi distribution in the cell was the same. Addition of choline instead of PC to the perfusate of compressed cells resulted in an accumulation of PC in the cytoplasmic compartment from choline kinase activity. In addition, PC phosphatase activity has been discovered associated with the cell wall. These results indicate that PC was rapidly hydrolyzed outside the cell and that choline and Pi entered the cytosolic compartment where choline kinase re-forms PC.

References

Feb 1, 1976·Journal of General Microbiology·T E Broad, R M Dawson
Apr 1, 1972·Analytical Biochemistry·E T Browning
Jun 1, 1974·Journal of Neurochemistry·G B Ansell, S G Spanner
Feb 1, 1966·Plant Physiology·K TanakaA F Gohlke
Dec 1, 1982·Archives of Biochemistry and Biophysics·F RebeilleR Douce
Nov 1, 1955·Plant Physiology·N E Tolbert, H Wiebe
Sep 1, 1956·Plant Physiology·J V MaizelN E Tolbert
May 1, 1986·Plant Physiology·A H Datko, S H Mudd

❮ Previous
Next ❯

Citations

Jan 15, 1993·European Journal of Biochemistry·N FertéG Noat
Jun 15, 2013·The Plant Journal : for Cell and Molecular Biology·Chiliang ChenGwyn A Beattie
Jul 23, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Juntao Liu, Yan Guo
May 13, 2004·Journal of Computational Chemistry·K FiatyB Maïsterrena
Jan 9, 1999·The Plant Journal : for Cell and Molecular Biology·M L NuccioA D Hanson
Aug 1, 1994·European Journal of Biochemistry·I Thiébart-Fassy, J F Hervagault

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.