Transport of poly-L-lysine into oppositely charged poly(acrylic acid) microgels and its effect on gel deswelling

Journal of Colloid and Interface Science
Helena BysellMartin Malmsten

Abstract

The interaction between poly-L-lysine (pLys) and oppositely charged poly(acrylic acid) (pAA) microgels (Ø approximately 80-120 microm) was studied by micromanipulator-assisted light microscopy and confocal laser scanning microscopy. The aim of this study was to investigate effects of peptide size, pH, and salt concentration on binding, transport, and distribution of pLys in pAA microgel particles and thereby also to outline the details of the gel deswelling in response to pLys binding and incorporation. Both peptide distribution and gel deswelling kinetics were found to be strongly influenced by the pLys molecular weight, originating partly from limited entry of large peptides into the gel particle core. Also pH was shown to influence both deswelling and pLys incorporation kinetics, with a decreased deswelling rate observed with increasing pH. These effects are determined by a complex interplay between the pH-dependence of both pLys and the gel network, also influencing volume transitions of the latter. Finally, salt concentration was shown to have a significant effect on both gel deswelling rate and pLys transport, with an increased electrolyte concentration resulting in decreased deswelling rate but also in an increased pepti...Continue Reading

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Citations

Feb 16, 2016·The Journal of Chemical Physics·J Gernandt, P Hansson
Jan 12, 2016·Biomacromolecules·Lina NyströmMartin Malmsten
Sep 15, 2011·Advanced Drug Delivery Reviews·Helena BysellMartin Malmsten
Apr 27, 2010·Journal of Colloid and Interface Science·Christian JohanssonPer Hansson
Aug 6, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Lynna ChenPatrick S Doyle
Jun 15, 2011·Journal of Colloid and Interface Science·Aldo LaganàMaria Vittoria Russo
Jan 9, 2015·The Journal of Physical Chemistry. B·Jonas Gernandt, Per Hansson
Dec 14, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Yuan LiJ Mieke Kleijn
Oct 4, 2012·The Journal of Physical Chemistry. B·Vindya K ThilakarathneChalla V Kumar
Jun 5, 2013·Biomacromolecules·Ronja MånssonMartin Malmsten
Nov 27, 2008·Journal of Biomaterials Applications·D SilvestriP Giusti
Nov 21, 2014·Materials·Ana M Díez-Pascual, Peter S Shuttleworth
May 4, 2020·Colloids and Surfaces. B, Biointerfaces·Xixi XiaoMatthew Libera
Dec 9, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Helena Bysell, Martin Malmsten
May 13, 2010·The Journal of Physical Chemistry. B·Helena BysellMartin Malmsten
Jul 9, 2009·Biomacromolecules·Helena BysellMartin Malmsten
Jan 6, 2010·The Journal of Physical Chemistry. B·Helena BysellMartin Malmsten
Apr 16, 2009·The Journal of Physical Chemistry. B·Christian JohanssonMartin Malmsten
Sep 5, 2009·The Journal of Physical Chemistry. B·Per Hansson
May 5, 2021·Accounts of Chemical Research·Jing LiangMatthew Libera
Feb 12, 2017·Accounts of Chemical Research·Felix A Plamper, Walter Richtering
Dec 25, 2010·Biomacromolecules·Ronja MånssonMartin Malmsten
Jun 28, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Jing LiangMatthew Libera
Jul 7, 2018·Biomacromolecules·Lina NyströmMartin Malmsten

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