PMID: 9437249Oct 1, 1994Paper

Transport of transferrin across the blood-thymus barrier in young rats

Tissue & Cell
R L Roberts, A Sandra

Abstract

The interaction of transferrin-peroxidase (Tf-HRP) with the capillary endothelium of the rat thymus was analyzed by diaminobenzidine (DAB) cytochemistry and electron microscopy. The thoracic aortas of young rats were cannulated and the upper bodies perfused with the Tf-HRP conjugate. In the thymus, plasmalemmal vesicles of the endothelium mediated the endocytosis and transport of Tf-HRP. Transcytosis of Tf-HRP appeared to occur by micropinocytosis, without morphological evidence of involvement by endothelial endosomes. DAB reaction product was commonly present in the subendothelial interstitial space and, in addition, was often localized in clathrin coated pits and vesicles in epithelial reticular cells that surround the thymic capillaries. In perfusions done at 4 degrees C, no binding of Tf-HRP to the lumenal membranes of capillaries was detected. The transport of Tf-HRP across the capillary endothelium in the thymus was not inhibited by competition with excess native transferrin; however, the uptake of Tf-HRP by epithelial reticular cells was completely inhibited by excess transferrin. Transferrin receptors were localized in the thymus by indirect immunocytochemistry using OX-26, a mouse anti-rat transferrin receptor monoclon...Continue Reading

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Citations

Oct 7, 2008·Cell and Tissue Research·Maya SimionescuAnca Sima
Jun 1, 2000·Experimental Gerontology·W PierpaoliS Arrighi
Jul 10, 2003·Autoimmunity Reviews·Christopher L Bowlus
Jul 12, 2002·Microscopy Research and Technique·Maya SimionescuFelicia Antohe
Nov 19, 2015·Biochimica Et Biophysica Acta·Damir PerisaArnold von Eckardstein
Apr 28, 2009·Progress in Neurobiology·Ivona BrasnjevicUNKNOWN European NanoBioPharmaceutics Research Initiative

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