PMID: 286Jan 1, 1975

Transport properties of membrane vesicles from Acholeplasma laidlawii. II. Kinetic characteristics and specificity of glucose transport system

Folia Microbiologica
L F PanchenkoM A Tarshis

Abstract

The glucose transport system of membrane vesicles isolated from Acholeplasma laidlawii is saturable, with a Km of 21.2 mum and V of 0.68 nmol min-1 (mg protein)-1. The process is pH-dependent and a break occurs in the Arrhenius plot at 15 degrees C. Exogenous substrates did not stimulate glucose transport probably due to their inability to penetrate into membrane vesicles. 3-O-Methylglucose and 6-deoxyglucose competitively inhibited glucose transport. Maltose inhibited transport of glucose noncompetitively. These sugars also elicited glucose efflux from preloaded membrane vesicles.

References

Oct 25, 1973·Biochimica Et Biophysica Acta·G van Thienen, P W Postma
May 26, 1972·Biochemical and Biophysical Research Communications·G D Sprott, R A MacLeod
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W N Konings, H R Kaback
Dec 3, 1973·European Journal of Biochemistry·M A TarshisH I Bourd
Aug 1, 1972·Journal of Bacteriology·P J Van Demark, P Plackett
Feb 1, 1969·Journal of Bacteriology·S Rottem, S Razin
Jul 15, 1969·Biochimica Et Biophysica Acta·H W Cho, H J Morowitz

Related Concepts

Acholeplasma laidlawii
Plasma Membrane
Deoxyglucose
Anhydrous Dextrose
Hydrogen-Ion Concentration
Maltose
Methylglucosides
Subcellular Fractions

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