Transposable element insertions shape gene regulation and melanin production in a fungal pathogen of wheat

BMC Biology
Parvathy KrishnanAndrea Sánchez-Vallet

Abstract

Fungal plant pathogens pose major threats to crop yield and sustainable food production if they are highly adapted to their host and the local environment. Variation in gene expression contributes to phenotypic diversity within fungal species and affects adaptation. However, very few cases of adaptive regulatory changes have been reported in fungi and the underlying mechanisms remain largely unexplored. Fungal pathogen genomes are highly plastic and harbor numerous insertions of transposable elements, which can potentially contribute to gene expression regulation. In this work, we elucidated how transposable elements contribute to variation in melanin accumulation, a quantitative trait in fungi that affects survival under stressful conditions. We demonstrated that differential transcriptional regulation of the gene encoding the transcription factor Zmr1, which controls expression of the genes in the melanin biosynthetic gene cluster, is responsible for variation in melanin accumulation in the fungal plant pathogen Zymoseptoria tritici. We show that differences in melanin levels between two strains of Z. tritici are due to two levels of transcriptional regulation: (1) variation in the promoter sequence of Zmr1 and (2) an inserti...Continue Reading

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Sep 26, 2019·Molecular Biology and Evolution·Simone FouchéDaniel Croll
Jan 15, 2020·Applied Microbiology and Biotechnology·Leandro Lopes da SilvaMarisa Vieira de Queiroz
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May 16, 2021·Fungal Genetics and Biology : FG & B·Benoit LaurentMarie Foulongne-Oriol

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Datasets Mentioned

BETA
SRP143580
PRJNA178194

Methods Mentioned

BETA
environmental stress
genetic modifications
restriction
PCR
RNA-seq
RNAseq
reverse transcription PCRs
Illumina sequencing

Software Mentioned

Excel
AliView
LightCycler 480
qtl
RStudio
R package genoPlotR
R
HTSeq
blastn
RepeatMasker

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