PMID: 8598301Mar 15, 1996Paper

Transposase is the only nematode protein required for in vitro transposition of Tc1

Genes & Development
J C VosR H Plasterk

Abstract

The Tc1 element of Caenorhabditis elegans is a member of the most widespread class of DNA transposons known in nature. Here, we describe efficient and precise transposition of Tc1 in a cell-free system. Tc1 appears to jump by a cut-and-paste mechanism of transposition. The terminal 26 bp of the Tc1 terminal repeats together with the flanking TA sequence are sufficient for transposition. The target site choice in vitro is similar to that in vivo. Transposition is achieved with an extract prepared from nuclei of transgenic nematodes that overexpress Tc1 transposase but also by recombinant transposase purified from Escherichia coli. The simple reaction requirements explain why horizontal spread of Tc1/mariner transposons can occur. They also suggest that Tcl may be a good vector for transgenesis of diverse animal species.

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A Shapiro
Oct 11, 1990·Nucleic Acids Research·E R Zabarovsky, G Winberg
Mar 20, 1988·Journal of Molecular Biology·D C RioG M Rubin
Jan 1, 1983·Cell·S W EmmonsD Katzenberg
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Bolland, N Kleckner
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·E L BeallD C Rio
Oct 21, 1994·Cell·H G van LuenenR H Plasterk
Sep 28, 1994·Molecular & General Genetics : MGG·A D RadiceS W Emmons
Feb 11, 1994·Nucleic Acids Research·H G van Luenen, R H Plasterk
Mar 1, 1994·Trends in Genetics : TIG·R GrosschedlJ Pagel
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·T G DoakG Herrick
Jan 1, 1996·Current Topics in Microbiology and Immunology·R H Plasterk

❮ Previous
Next ❯

Citations

May 8, 2003·Molecular Genetics and Genomics : MGG·O LadendorfJ Kämper
Feb 6, 2008·Molecular Genetics and Genomics : MGG·Roberta MoschettiRuggiero Caizzi
Dec 30, 2008·Molecular Genetics and Genomics : MGG·Gwénaëlle CrénèsAgnès Petit
Apr 7, 2009·Genetica·Valérie J Robert, Jean-Louis Bessereau
Jul 25, 2009·Genetica·Gwénaelle CrénèsSylvaine Renault
Oct 23, 2009·Genetica·Xavier ThomasSylvaine Renault
Dec 4, 2003·Gene·C GrabherM Furutani-Seiki
Jun 27, 2002·Journal of Molecular Biology·Zongbin CuiPerry B Hackett
Apr 1, 2000·Molecular and Biochemical Parasitology·R HoekstraM H Roos
Aug 4, 1999·Trends in Genetics : TIG·R H PlasterkZ Ivics
Oct 7, 1998·Epilepsy Research·H XieN C de Lanerolle
Dec 1, 1996·Current Opinion in Genetics & Development·A F Smit
Jul 17, 1998·Current Opinion in Structural Biology·J Jurka
Sep 25, 2003·Insect Biochemistry and Molecular Biology·K MichelP W Atkinson
Sep 1, 1996·Insect Biochemistry and Molecular Biology·H M Robertson, M L Asplund
Feb 13, 2001·Current Opinion in Chemical Biology·L HamerJ E Hamer
Feb 3, 1998·Nature Biotechnology·A Dawson, D J Finnegan
Apr 1, 1996·Nature Genetics·H M RobertsonD L Hartl
Sep 17, 2004·Molecular Biology and Evolution·Joana C SilvaJane M Carlton
Oct 10, 1997·Nucleic Acids Research·R F KettingR H Plasterk
Oct 10, 1997·Nucleic Acids Research·R RezsohazyR H Plasterk
Jun 5, 1998·Nucleic Acids Research·G J SchoutenR H Plasterk
Aug 28, 2001·Nucleic Acids Research·L ZhangD J Finnegan
Aug 12, 2004·Nucleic Acids Research·Stephan WatkinsTitia K Sixma
Nov 26, 2002·Molecular Biology and Evolution·Charles ClaudianosSteven Whyard
Aug 15, 1997·Genes & Development·E L Beall, D C Rio
Oct 17, 2006·The Plant Cell·Guojun YangSusan R Wessler
Jun 23, 2004·Development, Growth & Differentiation·Terumi MatsuokaYasunori Sasakura
May 11, 2010·Applied and Environmental Microbiology·Jaroslaw E KrólEva M Top
Aug 24, 2002·Journal of Bacteriology·Akihiro UrasakiEiichi Ohtsubo
Mar 4, 2005·Molecular and Cellular Biology·Stephen R YantMark A Kay
Jan 1, 1997·Annual Review of Biochemistry·N L Craig
Jan 1, 1997·Annual Review of Genetics·D L HartlE R Lozovskaya
Jan 1, 1997·Annual Review of Genetics·M Labrador, V G Corces

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.