Transposition of a bacterial insertion sequence in chloroplasts

The Plant Journal : for Cell and Molecular Biology
Stefan Kohl, Ralph Bock

Abstract

Bacterial transposable elements (IS elements, transposons) represent an important determinant of genome structure and dynamics, and are a major force driving genome evolution. Here, we have tested whether bacterial insertion sequences (IS elements) can transpose in a prokaryotic compartment of the plant cell, the plastid (chloroplast). Using plastid transformation, we have integrated different versions of the Escherichia coli IS element IS150 into the plastid genome of tobacco (Nicotiana tabacum) plants. We show that IS150 is faithfully mobilized inside the chloroplast, and that enormous quantities of transposition intermediates accumulate. As synthesis of the IS150 transposase is dependent upon programmed ribosomal frame shifting, our data indicate that this process also occurs in chloroplasts. Interestingly, all insertion events detected affect a single site in the plastid genome, suggesting that the integration of IS150 is highly sequence dependent. In contrast, the initiation of the transposition process was found to be independent of the sequence context. Finally, our data also demonstrate that plastids lack the capacity to repair double-strand breaks in their genomes by non-homologous end joining, a finding that has impor...Continue Reading

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Citations

Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Taegun KwonDavid L Herrin
Oct 19, 2010·Biochimica Et Biophysica Acta·Pierre BoeschAndré Dietrich
Jun 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Neil H KimThomas E Kuhlman
Jul 31, 2016·BMC Plant Biology·Elena Martin AvilaAnil Day
Jul 10, 2019·Nature Plants·Tomohiko KazamaShin-Ichi Arimura
Jun 14, 2015·The Plant Cell·Masato Nakai

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