PMID: 375023Mar 9, 1979Paper

Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element

Molecular & General Genetics : MGG
R MeyerJ Shapiro

Abstract

Tn5-trp hybrid transposons have been constructed by insertion of a trpPOED Hind III fragment into an in vivo Tn5 internal deletion mutant or by substitution of trp for the internal Tn5 Hind III fragment. These hybrids are called, respectively, Tn409 and Tn410. Both Tn409 and Tn410 will transpose into lambda in the presence of a complementing Tn5 element. In the absence of a wild Tn5, lysogens carrying R1162::Tn409 and R1162::Tn410 plasmids will yield lambdatrp phages at less than six per cent of the complemented frequency. This reduction indicates that Tn409 and Tn410 lack a diffusible transposition function provided by wild Tn5 elements. However, the formation of lambdatrp phages without complementation is real. Most of these transducing particles contain Tn409 and Tn410 still linked to the carrier R1162 plasmid. This observation suggests that uncomplemented Tn409 and Tn410 elements mediate the formation of lambda-transposon-plasmid cointegrate structures. Thus, the missing transposition function may be involved in resolving these cointegrate structures to the final lambda::Tn409 or lambda::Tn410 product.

References

Feb 1, 1978·Plasmid·M FennewaldJ Shapiro
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·J A Shapiro, L A MacHattie
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·N D Grindley, D J Sherratt
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L A MacHattie, J A Shapiro
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F HeffronS Falkow
Apr 29, 1977·Molecular & General Genetics : MGG·R MeyerD R Helinski
Sep 1, 1978·Journal of Virology·B T Waggoner, M L Pato
Jul 5, 1976·Journal of Molecular Biology·M Faelen, A Toussaint
Jan 1, 1976·Annual Review of Genetics·A I Bukhari
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D E BergJ D Rochaix
Jul 10, 1975·Nature·N Sternberg, R Weisberg
Nov 14, 1975·Science·M M Howe, E G Bade
Mar 7, 1973·Nature: New Biology·A Toussaint, M Faelen

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Citations

Jan 1, 1981·Molecular & General Genetics : MGG·T S LlyinaG B Smirnov
Jan 1, 1981·Molecular & General Genetics : MGG·T S IlyinaG B Smirnov
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R R ReedM S Guyer
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D Biek, J R Roth
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G ChaconasA I Bukhari
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D E Berg
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C Egner, D E Berg
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P KittsD Sherratt
Jun 1, 1983·Cell·T J ZupancicS R Jaskunas
Jan 1, 1980·Microbiology and Immunology·T YamamotoS Yamagishi
May 1, 1980·Journal of Bacteriology·D E BergL Crossland
May 1, 1980·Journal of Bacteriology·N J CrisonaA J Clark
Jul 1, 1979·Journal of Bacteriology·M A Fennewald, J A Shapiro
Jul 1, 1980·Cell·M P Calos, J H Miller
Dec 15, 1980·Journal of Molecular Biology·D A FischhoffN D Zinder
Feb 25, 1982·Journal of Molecular Biology·B J Hirschel, D E Berg
Sep 19, 1980·Science·G S RoederG R Fink

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