Transposition of the heat-stable toxin astA gene into a gifsy-2-related prophage of Salmonella enterica serovar Abortusovis

Journal of Bacteriology
Donatella BacciuSergio Uzzau

Abstract

The horizontal transfer and acquisition of virulence genes via mobile genetic elements have been a major driving force in the evolution of Salmonella pathogenicity. Serovars of Salmonella enterica carry variable assortments of phage-encoded virulence genes, suggesting that temperate phages play a pivotal role in this process. Epidemic isolates of S. enterica serovar Typhimurium are consistently lysogenic for two lambdoid phages, Gifsy-1 and Gifsy-2, carrying known virulence genes. Other serovars of S. enterica, including serovars Dublin, Gallinarum, Enteritidis, and Hadar, carry distinct prophages with similarity to the Gifsy phages. In this study, we analyzed Gifsy-related loci from S. enterica serovar Abortusovis, a pathogen associated exclusively with ovine infection. A cryptic prophage, closely related to serovar Typhimurium phage Gifsy-2, was identified. This element, named Gifsy-2AO, was shown to contribute to serovar Abortusovis systemic infection in lambs. Sequence analysis of the prophage b region showed a large deletion which covers genes encoding phage tail fiber proteins and putative virulence factors, including type III secreted effector protein SseI (GtgB, SrfH). This deletion was identified in most of the serovar...Continue Reading

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Citations

Oct 10, 2013·Foodborne Pathogens and Disease·Federico CapuanoYolande T R Proroga
Dec 15, 2007·The New Phytologist·Honour C McCann, David S Guttman
Jan 30, 2013·Critical Reviews in Microbiology·Mehari Tesfazgi MebrhatuAbram Aertsen
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May 17, 2017·Biophysics Reviews·N Patrick Higgins
Dec 9, 2021·Current Microbiology·Giulia AmaglianiEnrica Omiccioli

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