Transposon-based activation tagging in cereals

Functional Plant Biology : FPB
Michael A Ayliffe, Anthony J Pryor

Abstract

Advances in DNA sequencing technologies have produced an ever increasing number of sequenced genomes. However, many of the genes identified in these sequencing efforts have unknown functions or functions inferred based upon sequence homology, highlighting the necessity for functional gene analysis. Mutagenesis combined with phenotypic analyses remains a key mechanism for identifying and establishing gene function. Activation tagging is a mutagenic process that uses altered gene expression, usually gene overexpression, to generate mutant phenotypes. We have developed an activation tagging system in barley (Hordeum vulgare L.) based upon a maize (Zea mays L.) transposable element that carries two highly expressed cereal promoters. Insertion of this mobile genetic element in the genome can lead to insertional gene inactivation, gene overexpression and gene silencing through the production of antisense transcripts. This transposable element system has also been introduced into both wheat (Triticum aestivum L.) and maize and transposon mobility observed.

References

Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·P Rørth
Apr 12, 2000·Plant Physiology·D WeigelJ Chory
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·J S JeonG An
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·T KoprekP G Lemaux
Feb 24, 2001·Molecular & General Genetics : MGG·S ScholzS Lütticke
Mar 3, 2001·Current Opinion in Plant Biology·N Bouché, D Bouchez
Mar 3, 2001·Current Opinion in Plant Biology·H Hirochika
Aug 15, 2002·Plant Physiology·Nayelli Marsch-MartinezAndy Pereira
Dec 14, 2002·Plant Physiology·Dong-Hoon JeongGynheung An
May 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·L YanJ Dubcovsky
Jul 31, 2003·The Plant Journal : for Cell and Molecular Biology·Changyin WuQifa Zhang
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Sep 17, 2003·The Plant Journal : for Cell and Molecular Biology·Shuangyan ChenPing Wu
Dec 24, 2003·The Plant Journal : for Cell and Molecular Biology·Tatiana KolesnikSrinivasan Ramachandran
Feb 6, 2004·The Plant Journal : for Cell and Molecular Biology·Nabila YahiaouiBeat Keller
May 25, 2004·Functional & Integrative Genomics·Helen TaniGary J Loake
Jun 29, 2004·Molecular Genetics and Genomics : MGG·Y ItoN Kurata
Jul 1, 2004·The Plant Journal : for Cell and Molecular Biology·Chul Min KimChang-deok Han
Jul 17, 2004·The Plant Journal : for Cell and Molecular Biology·Christophe SallaudEmmanuel Guiderdoni
Sep 28, 2004·Molecular Genetics and Genomics : MGG·L D CooperP M Hayes
Dec 22, 2005·The Plant Journal : for Cell and Molecular Biology·Dong-Hoon JeongGynheung An
Dec 31, 2005·Nucleic Acids Research·Jianwei ZhangShiping Wang
Aug 8, 2006·The Plant Journal : for Cell and Molecular Biology·Tiehan ZhaoThomas Koprek
Nov 23, 2006·Plant Molecular Biology·Yue-Ie HsingSu-May Yu
Aug 1, 1987·Molecular & General Genetics : MGG·N TheresP Starlinger
Apr 13, 2007·Plant Molecular Biology·Michael A AyliffeAnthony J Pryor
Sep 25, 2007·The New Phytologist·Chuanzao MaoPing Wu
Oct 20, 2007·Nucleic Acids Research·Pierre LarmandeEmmanuel Guiderdoni
Dec 28, 2007·Nature Reviews. Genetics·Ki-Hong JungPamela C Ronald
Oct 3, 2008·Plant Molecular Biology·Shuyan WanTiegang Lu
Jan 8, 2009·Plant Physiology·Clifford F Weil
May 1, 2002·Functional Plant Biology : FPB·Narayana M UpadhyayaElizabeth S Dennis

❮ Previous
Next ❯

Citations

May 25, 2021·Journal of Advanced Research·M Raghurami ReddyS M Balachandran

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Methods in Molecular Biology
Michael A Ayliffe, Anthony J Pryor
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
S IidaK Shimamoto
Nature Biotechnology
Jeffrey F D Dean
Trends in Plant Science
T MaesT Gerats
© 2021 Meta ULC. All rights reserved