PMID: 8610121Apr 2, 1996Paper

Transposon tools for recombinant DNA manipulation: characterization of transcriptional regulators from yeast, Xenopus, and mouse

Proceedings of the National Academy of Sciences of the United States of America
B A MorganS G Sedgwick

Abstract

Transposon Tn1000 has been adapted to deliver novel DNA sequences for manipulating recombinant DNA. The transposition procedure for these "tagged" Tn1000s is simple and applicable to most plasmids in current use. For yeast molecular biology, tagged Tn1000s introduce a variety of yeast selective markers and replication origins into plasmids and cosmids. In addition, the beta-globin minimal promoter and lacZ gene of Tn(beta)lac serve as a mobile reporter of eukaryotic enhancer activity. In this paper, Tn(beta)lac was used to localize a mouse HoxB-complex enhancer in transgenic mice. Other tagged transposons create Gal4 DNA-binding-domain fusions, in either Escherichia coli or yeast plasmids, for use in one- and two-hybrid tests of transcriptional activation and protein-protein interaction, respectively. With such fusions, the Saccharomyces cerevisiae Swi6 G1/S-phase transcription factor and the Xenopus laevis Pintallavis developmental regulator are shown to activate transcription. Furthermore, the same transposon insertions also facilitated mapping of the Swi6 and Pintallavis domains responsible for transcriptional activation. Thus, as well as introducing novel sequences, tagged transposons share the numerous other applications o...Continue Reading

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Citations

Jul 17, 2008·Comparative and Functional Genomics·Jo Wixon
Dec 7, 2010·Transgenic Research·Junko AsamiTakayoshi Inoue
Apr 12, 2013·Human Molecular Genetics·Jessica M RosinJohn Cobb
Oct 6, 2000·The Journal of Cell Biology·H BrowningJ R McIntosh
Jul 12, 2008·Molecular and Cellular Neurosciences·Yukiko U InoueTakayoshi Inoue
Mar 1, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Stuart J SmithTimothy J Mohun
Feb 27, 2008·The Plant Journal : for Cell and Molecular Biology·Frank HochholdingerPatrick S Schnable
Jul 7, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Stuart J SmithTimothy J Mohun
Apr 27, 2000·Mechanisms of Development·Y SakaJ C Smith
Aug 10, 2016·Scientific Reports·Yukiko U Inoue, Takayoshi Inoue
Aug 28, 1998·Journal of Molecular Biology·S G SedgwickS J Smerdon
Aug 30, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Linda S KaltenbachSusan E Mango
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katharine E MagorPeter Parham

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