Transrectal ultrasound for monitoring murine orthotopic prostate tumor

The Prostate
N KusakaHiromi Kumon

Abstract

The mouse orthotopic prostate tumor model has been recognized as an ideal preclinical animal model simulating the anatomical and biological milieu of the prostate. In comparison with the subcutaneous tumor model, the only disadvantage of this model is the difficulty of chronological tumor growth monitoring. We have applied recent endoluminal ultrasound technology, transrectal ultrasonography (TRUS), to the monitoring of mouse orthotopic prostate tumors. A 6 Fr. 20 MHz catheter-based radial scan probe was used and TRUS was performed without any prior preparation including anesthesia. Orthotopic tumors were initiated by inoculation of 5000 RM-9 cells into the dorsal prostate of 12-week-old C57BL/6 male mice. The tumor growth was monitored by TRUS from day 3 to day 21. In addition, TRUS was performed to detect tumor growth suppression after intraperitoneal administration of cis-diamminedichloroplatinum (CDDP). By ultrasound, tumors became detectable 7 days after tumor cell inoculation. TRUS images were clear and parallel to actual tumor growth. The tumor volume (X) calculated by TRUS correlated significantly with the actual tumor weight (Y) measured at autopsy; Y = 101.653 + 1.174X (R = 0.930, P < 0.001). Similarly, tumor growth s...Continue Reading

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Citations

May 29, 2012·Ultrasound in Medicine & Biology·Naoki HiraharaTsuneharu Miki
Jan 8, 2008·Chemico-biological Interactions·Wytske M van Weerden, Fritz H Schröder
Feb 9, 2006·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·John C PezoldEben L Rosenthal
Apr 15, 2006·The Journal of Nutrition·Jacqueline LimpensWytske M van Weerden

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