Transscleral Iontophoresis for Noninvasive Ocular Drug Delivery of Macromolecules.

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
Sarah MolokhiaWilliam I Higuchi

Abstract

Purpose: The objectives were to investigate the effect of transscleral iontophoresis of macromolecules in vitro and in vivo, to study the importance of electroosmosis on macromolecules of low charge to mass ratio, and to evaluate transscleral iontophoresis efficacy in a choroidal neovascularization (CNV) animal model. Methods: Through in vitro transport experiments, the permeability coefficients of macromolecules [eg, immunoglobulin G (IgG), dextran 70 kDa] were determined under different conditions. The effect of ionic strength formulations and iontophoretic conditions was studied on the distribution of IgG and bevacizumab into the eye in vivo. Magnetic resonance imaging (MRI) was utilized to evaluate in vivo real time distribution of gadolinium-labeled albumin (Galbumin) following iontophoresis. The efficacy between no treatment, intravitreal injection (IVT), and iontophoresis of bevacizumab on a CNV model of subretinal injection of adeno-associated virus encoding human VEGF-165 was investigated. Results: The permeability data suggested a significant effect of ionic strength on the iontophoretic transport of macromolecules. Transscleral iontophoresis of IgG at 4 mA with a low ionic strength formulation was about 600 times gre...Continue Reading

References

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Oct 13, 2017·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Kongnara PapangkornWilliam I Higuchi
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Jul 12, 2018·Molecular Pharmaceutics·Mariya A PindrusDevendra S Kalonia

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Citations

Jan 21, 2021·Pharmaceutics·Hyeong Min Kim, Se Joon Woo
Jul 5, 2021·Ophthalmology and Therapy·Lixiang WangHui Zhang
Aug 18, 2021·International Journal of Pharmaceutics·Katarzyna B WróblewskaIzabela Muszalska-Kolos

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Methods Mentioned

BETA
electrophoresis
enzyme-linked immunosorbent assay
ELISA
imaging technique
dynamic light scattering

Software Mentioned

MRIcro
ImageJ

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