Transscleral visible/near-infrared spectroscopy for quantitative assessment of melanin in a uveal melanoma phantom of ex vivo porcine eyes

Experimental Eye Research
Jørgen KrohnStefan Andersson-Engels

Abstract

Optical spectroscopy has been used as a supplement to conventional techniques for analyzing and diagnosing cancer in many human organs. Because ocular tumors may be characterized by their different melanin content, we investigated the feasibility of using transscleral visible/near-infrared spectroscopy (Vis/NIRS) to estimate the quantity of melanin in a novel uveal melanoma phantom of ex vivo porcine eyes. The phantoms were made by injecting a freshly prepared suspension of 15% (wt/vol) gelatin, 10 mg/ml titanium dioxide (TiO(2)), and natural melanin, isolated from the ink sac of cuttlefish (Sepia officinalis), into the suprachoroidal space of 30 enucleated porcine eyes. The melanin concentrations used were 1 mg/ml, 2 mg/ml, and 3 mg/ml, with 10 eyes in each group. After gelation, the size and location of the phantoms were documented by B-scan ultrasonography and transillumination. Vis/NIRS recordings, covering the wavelength region from 550 to 1000 nm, were performed with two optical fibers separated by 6 mm to deliver and collect the light through the sclera. During all measurements, the exact pressure exerted by the fiber probe on the scleral surface was monitored by placing the eye on an electronic scale. Transscleral Vis/N...Continue Reading

References

Jan 1, 1991·Lasers in Surgery and Medicine·A VogelR Birngruber
Jun 1, 1995·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·C E Margo, A Lee
Jan 1, 1996·Annual Review of Physical Chemistry·R Richards-Kortum, E Sevick-Muraca
Feb 19, 1998·American Journal of Ophthalmology·T W OlsenH F Edelhauser
Jun 5, 1998·The British Journal of Ophthalmology·B SobottkaI Kreissig
Nov 24, 1998·Acta Ophthalmologica Scandinavica·J Krohn, T Bertelsen
Nov 5, 1999·Human Reproduction·R HornungB J Tromberg
Feb 13, 2001·The Journal of Investigative Dermatology·L M McIntoshJ W Toole
Feb 12, 2002·Physics in Medicine and Biology·Martin Hammer, Dietrich Schweitzer
Jan 27, 2005·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·M SchneiderD Leupold
Mar 28, 2006·Biophysical Journal·Jennifer RieszPaul Meredith
Sep 13, 2006·Journal of Biomedical Optics·Brian W Pogue, Michael S Patterson
Oct 27, 2006·Investigative Ophthalmology & Visual Science·Sveinn Hakon HardarsonEinar Stefánsson
Jan 6, 2007·Analytical and Bioanalytical Chemistry·Venkata Radhakrishna KondepatiJuergen Backhaus
Mar 9, 2007·Journal of Biomedical Optics·Tomas SvenssonKatarina Svanberg
Jul 2, 2009·Journal of Biomedical Optics·Reinhold EichhornKlaus Hoffmann

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Citations

Mar 28, 2013·The British Journal of Ophthalmology·Jørgen KrohnBård Kjersem
Nov 15, 2011·Biomedical Optics Express·Pontus SvenmarkerJørgen Krohn

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