Transverse electric field dragging of DNA in a nanochannel.

Scientific Reports
Makusu TsutsuiTomoji Kawai

Abstract

Nanopore analysis is an emerging single-molecule strategy for non-optical and high-throughput DNA sequencing, the principle of which is based on identification of each constituent nucleobase by measuring trans-membrane ionic current blockade or transverse tunnelling current as it moves through the pore. A crucial issue for nanopore sequencing is the fact that DNA translocates a nanopore too fast for addressing sequence with a single base resolution. Here we report that a transverse electric field can be used to slow down the translocation. We find 400-fold decrease in the DNA translocation speed by adding a transverse field of 10 mV/nm in a gold-electrode-embedded silicon dioxide channel. The retarded flow allowed us to map the local folding pattern in individual DNA from trans-pore ionic current profiles. This field dragging approach may provide a new way to control the polynucleotide translocation kinetics.

References

Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J KasianowiczD W Deamer
Jul 23, 2004·The Journal of Chemical Physics·Shunsaku TakeishiNaoki Yokoyama
Sep 17, 2004·Journal of the American Chemical Society·Hiromi Kimura-SudaLloyd J Whitman
Sep 15, 2005·Nano Letters·Daniel FologeaJiali Li
Oct 13, 2005·Nano Letters·Daniel FologeaJiali Li
Apr 13, 2006·Nano Letters·Johan LagerqvistMassimiliano Di Ventra
May 5, 2007·The Journal of Chemical Physics·C T A Wong, M Muthukumar
May 29, 2007·Biophysical Journal·Johan LagerqvistMassimiliano Di Ventra
Mar 15, 2008·Biophysical Journal·Christian H RecciusH G Craighead
Jul 26, 2008·Nature Nanotechnology·Cees Dekker
Oct 11, 2008·Nature Biotechnology·Daniel BrantonJeffery A Schloss
Apr 8, 2009·Nature Nanotechnology·James ClarkeHagan Bayley
Mar 23, 2010·Nature Nanotechnology·Makusu TsutsuiTomoji Kawai
Jun 3, 2010·Nano Letters·Lorenz J SteinbockUlrich F Keyser
Jul 9, 2010·Nano Letters·Grégory F SchneiderCees Dekker
Aug 20, 2010·Nature·S GarajJ A Golovchenko
Aug 24, 2010·Nature Chemistry·Matthias ErdmannHermann E Gaub
Sep 2, 2010·Analytical Chemistry·Ye AiShizhi Qian
Nov 16, 2010·Nature Nanotechnology·Shuo HuangStuart Lindsay
Dec 3, 2010·Journal of the American Chemical Society·Kate R LiebermanMark Akeson
Dec 8, 2010·Nano Letters·Aleksandar P IvanovJoshua B Edel
Feb 22, 2011·Nature Nanotechnology·Erik C YuskoMichael Mayer
Feb 23, 2012·Scientific Reports·Makusu TsutsuiTomoji Kawai

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Citations

Aug 15, 2014·International Journal of Molecular Sciences·Weixin QianSatoyuki Kawano
Feb 24, 2016·Physical Chemistry Chemical Physics : PCCP·Lanju MeiShizhi Qian
May 2, 2015·Electrophoresis·Matthew WaughVincent Tabard-Cossa
Feb 4, 2016·Nature Nanotechnology·Massimiliano Di Ventra, Masateru Taniguchi
Aug 17, 2016·Scientific Reports·Shoji TanakaTomoji Kawai
Nov 10, 2012·Electrophoresis·Raj D MaitraWilliam B Dunbar
Aug 20, 2014·Physical Chemistry Chemical Physics : PCCP·Yu MaHeping Tan
Nov 3, 2017·Advanced Materials·Yan Xu
Apr 15, 2018·Scientific Reports·Sara MahshidMohammed Jalal Ahamed
Apr 24, 2019·Sensors·Qi Chen, Zewen Liu
Mar 4, 2019·European Biophysics Journal : EBJ·Han YanDeqiang Wang
Oct 4, 2019·Reviews of Modern Physics·Subin Sahu, Michael Zwolak
Jun 11, 2015·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Maria Fyta
May 8, 2014·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Kentaro DoiMassimiliano Di Ventra
Aug 21, 2021·Biosensors & Bioelectronics·Yuan WangQuan Qing
May 10, 2018·ACS Applied Materials & Interfaces·Sang Won JungYong-Hoon Kim
Mar 26, 2014·Nanoscale Research Letters·Lingzhi WuZuhong Lu

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Methods Mentioned

BETA
fluorescence microscopy

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