PMID: 9547287Jun 20, 1998Paper

Transverse formamide gradients as a simple and easy way to optimise DNA single-strand conformation polymorphism analysis

Nucleic Acids Research
B PaccoudR Sesboüé

Abstract

Although widely used, the detection of DNA mutations by the single-strand conformation polymorphism (SSCP) method is often hampered by the need to examine a large set of electrophoretic conditions in order to select the one suited to the DNA sequence under study. We show here that the use of transverse chemical gradient gels allows for a quick and easy optimisation of SSCP analysis, as exemplified on two mutations in exon 2 of the alpha-1-antitrypsin gene.

References

Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M OritaT Sekiya
Feb 1, 1996·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·W TeschauerE Fink

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