PMID: 7544009Aug 15, 1995Paper

TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis, is a toroid-shaped molecule that binds transcripts containing GAG or UAG repeats separated by two nucleotides

Proceedings of the National Academy of Sciences of the United States of America
Paul BabitzkeCharles Yanofsky

Abstract

The trp RNA-binding attenuation protein of Bacillus subtilis, TRAP, regulates both transcription and translation by binding to specific transcript sequences. The optimal transcript sequences required for TRAP binding were determined by measuring complex formation between purified TRAP protein and synthetic RNAs. RNAs were tested that contained repeats of different trinucleotide sequences, with differing spacing between the repeats. A transcript containing GAG repeats separated by two-nucleotide spacers was bound most tightly. In addition, transmission electron microscopy was used to examine the structure of TRAP and the TRAP-transcript complex. TRAP was observed to be a toroid-shaped oligomer when free or when bound to either a natural or a synthetic RNA.

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Citations

Jan 1, 1996·Biochimie·C YanofskyJ P Sarsero
Jun 1, 1997·Current Opinion in Structural Biology·J D GriffithY H Wang
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Masahiro WatanabeJeremy R H Tame
Jun 17, 2004·Nucleic Acids Research·S Hani Najafi-ShoushtariMichael Famulok
Jan 1, 1996·Annual Review of Genetics·T M Henkin
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y LuR L Switzer
Nov 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mikhail B ShevtsovAlfred A Antson
Mar 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·J P SarseroC Yanofsky
Jul 22, 2009·Journal of Molecular Biology·Jeffrey MercanteTony Romeo
Feb 22, 2008·Protein Science : a Publication of the Protein Society·Masahiro WatanabeJonathan G Heddle
Mar 19, 2015·Frontiers in Microbiology·Elke Van AsscheHans P Steenackers
Nov 5, 1999·Journal of Molecular Biology·D E Draper
Dec 14, 2004·Journal of Molecular Biology·Maria V BarbolinaPaul Gollnick
Jun 15, 2005·Trends in Genetics : TIG·A Gutierrez-PreciadoE Merino
Dec 1, 2017·Biophysics Reviews·Satoru Unzai
Jan 24, 2002·The Journal of Biological Chemistry·Pan T X LiPaul Gollnick
Aug 14, 1998·Molecular Microbiology·M van de GuchteA Chopin
May 19, 2019·Annual Review of Microbiology·Paul BabitzkeTony Romeo

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