TRAP150 activates pre-mRNA splicing and promotes nuclear mRNA degradation.

Nucleic Acids Research
Kuo-Ming LeeWoan-Yuh Tarn

Abstract

TRAP150 has been identified as a subunit of the transcription regulatory complex TRAP/Mediator, and also a component of the spliceosome. The exact function of TRAP150, however, remains unclear. We recently identified TRAP150 by its association with the mRNA export factor TAP. TRAP150 contains an arginine/serine-rich domain and has sequence similarity with the cell death-promoting transcriptional repressor BCLAF1. We found that TRAP150 co-localizes with splicing factors in nuclear speckles, and is required for pre-mRNA splicing and activates splicing in vivo. TRAP150 remains associated with the spliced mRNA after splicing, and accordingly, it interacts with the integral exon junction complex. Unexpectedly, when tethered to a precursor mRNA, TRAP150 can trigger mRNA degradation in the nucleus. However, unlike nonsense-mediated decay, TRAP150-mediated mRNA decay is irrespective of the presence of upstream stop codons and occurs in the nucleus. Moreover, TRAP150 activates pre-mRNA splicing and induces mRNA degradation by its separable functional domains. Therefore, TRAP150 represents a multi-functional protein involved in nuclear mRNA metabolism.

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Citations

Nov 2, 2012·Molecular Biology of the Cell·Tzu-Wei ChuangWoan-Yuh Tarn
Jan 13, 2012·Human Reproduction·K StouffsW Lissens
May 14, 2014·PloS One·Markus E DiefenbacherOlivier Kassel
Oct 26, 2013·International Journal of Cell Biology·Silvia C LenzkenSilvia M L Barabino
Oct 19, 2014·Nucleic Acids Research·Kuo-Ming Lee, Woan-Yuh Tarn
Nov 4, 2015·Biomolecules·Lulzim Shkreta, Benoit Chabot
Jan 17, 2016·Nucleic Acids Research·Agnieszka M GromadzkaNiels H Gehring
Apr 26, 2013·The Journal of Pathology·Katayoon KasaianSteven J M Jones
Dec 18, 2014·Wiley Interdisciplinary Reviews. RNA·Yan HuangGang Wang
Jul 16, 2014·Biochemical and Biophysical Research Communications·Tetsurou SatohMasanobu Yamada
Nov 22, 2014·Nucleic Acids Research·Shuo-Hsiu ChangWoan-Yuh Tarn
Jul 2, 2014·Journal of Proteome Research·Suliman A AlsagabyPaul Brennan
Feb 25, 2014·Molecular & Cellular Proteomics : MCP·Kathrine B SylvestersenMichael L Nielsen
Mar 28, 2018·Molecular and Cellular Biology·Dhananjaya DWoan-Yuh Tarn
Aug 5, 2017·Journal of Bone and Mineral Metabolism·Takashi SonoShuichi Matsuda
Jun 24, 2015·Molecular and Cellular Biology·Gina KavanaughDavid Cortez
Mar 26, 2013·Molecular Endocrinology·Akiko Katano-TokiMasatomo Mori
Jun 14, 2018·International Journal of Molecular Sciences·Kersti NilssonStefan Schwartz
Mar 30, 2018·Nucleic Acids Research·Kersti NilssonStefan Schwartz
Mar 6, 2021·Gene·Kiran Kumar Kolathur
Dec 19, 2017·Chemical Reviews·Xavier RamboutLynne E Maquat
Sep 17, 2021·Frontiers in Physiology·Zhenguo WangLin Chang

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BETA
PCR
immunoprecipitation
fluorescence microscopy
transfection
electrophoresis

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