Abstract
Long strands of DNA can be trapped and concentrated near the inlet of a microfluidic channel by applying a pressure gradient and an opposing electric field. The mechanism for trapping involves a migration of DNA perpendicular to both the fluid flow and the electric field. Migration leads to a highly nonuniform distribution of DNA within a cross section of the channel, with the bulk of the DNA concentrated in a thin (10 μm) layer next to the walls of the channel. This highly concentrated layer generates an electrophoretic flux toward the inlet to the device, despite the much larger fluid flow in the opposite direction. In this paper, the extent to which DNA can be trapped and concentrated by this means has been characterized by fluorescence measurements. At short times (<2 hours) nearly all the incoming DNA remains trapped within the device until the electric field is turned off. The DNA largely accumulates near the inlet, but after 30-60 minutes additional DNA starts to accumulate deeper into the channel. Eventually DNA leaks from the device itself, but ≈80% of the incoming DNA can be retained for up to 5 hours. Optimizing the electric field strength can increase the amount of DNA that can be trapped, but the efficiency is not ...Continue Reading
References
Apr 1, 1995·Electrophoresis·S C Jacobson, J M Ramsey
Jan 13, 1995·Science·L MitnikJ L Viovy
Sep 19, 2002·Analytical Chemistry·Jinjian Zheng, Edward S Yeung
Aug 9, 2003·Physical Review Letters·Richard M JendrejackJuan J de Pablo
Oct 24, 2003·Analytical Chemistry·Jinjian Zheng, Edward S Yeung
Jul 23, 2004·The Journal of Chemical Physics·Richard M JendrejackMichael D Graham
Mar 16, 2007·Physical Review Letters·O Berk UstaAnthony J C Ladd
Aug 26, 2010·Electrophoresis·Wei-Ching LiaoLy James Lee
Dec 13, 2012·The Analyst·Supreet S Bahga, Juan G Santiago
Apr 23, 2015·Soft Matter·Mert ArcaAnthony J C Ladd
Aug 21, 2015·Annual Review of Biomedical Engineering·Francis CuiAnubhav Tripathi
Dec 15, 2015·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Harsh Pandey, Patrick T Underhill
Jan 29, 2016·Lab on a Chip·Sarah M FriedrichTza-Huei Wang
Mar 5, 2016·Lab on a Chip·Hubert RanchonAurélien Bancaud
Jul 29, 2016·Soft Matter·Mert ArcaJason E Butler
Nov 2, 2017·Nature Communications·Sarah M FriedrichTza-Huei Wang
Mar 3, 2018·Analytical Chemistry·Comtet-Louis AndriamanampisoaAziz Zaanan
Citations
Mar 1, 2019·Electrophoresis·Xiangchun Xuan
Oct 19, 2019·Micromachines·Zheyun XuJing Tu
Jun 9, 2020·Soft Matter·Jeffrey TeilletAurélien Bancaud
Jul 2, 2020·The Analyst·Benjamin E ValleyAnthony J C Ladd
Jan 2, 2020·Biomicrofluidics·Ryan J MontesJason E Butler
Dec 25, 2019·Physical Review. E·Angelo C Setaro, Patrick T Underhill
Apr 17, 2020·Analytical Chemistry·Murat SerhatliogluCaglar Elbuken
Nov 19, 2021·Electrophoresis·Leilei Shi, Leyla Esfandiari