Traveling waves of in vitro evolving RNA.

Proceedings of the National Academy of Sciences of the United States of America
G J BauerH Otten

Abstract

Populations of short self-replicating RNA variants have been confined to one side of a reaction-diffusion traveling wave front propagating along thin capillary tubes containing the Q beta viral enzyme. The propagation speed is accurately measurable with a magnitude of about 1 micron/sec, and the wave persists for hundreds of generations (of duration less than 1 min). Evolution of RNA occurs in the wavefront, as established by front velocity changes and gel electrophoresis of samples drawn from along the capillary. The high population numbers (approximately equal to 10(11], their well-characterized biochemistry, their short generation time, and the constant conditions make the system ideal for evolution experiments. Growth is monitored continuously by excitation of an added RNA-sensitive fluorescent dye, ethidium bromide. An analytic expression for the front velocity is derived for the multicomponent kinetic scheme that reduces, for a high RNA-enzyme binding constant, to the Fisher form v = 2 square root of kappa D, where D is the diffusion constant of the complex and kappa is the low-concentration overall replication rate coefficient. The latter is confirmed as the selective value-determining parameter by numerical solution of ...Continue Reading

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Sumper, R Luce
Jan 1, 1987·Journal of Mathematical Biology·K Aoki
Jul 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·D R MillsS Spiegelman
Jul 3, 1984·Biochemistry·C K BiebricherW C Gardiner
Jun 5, 1981·Journal of Molecular Biology·C K BiebricherR Luce
May 10, 1983·Biochemistry·C K BiebricherW C Gardiner
Feb 5, 1982·Journal of Molecular Biology·C K BiebricherR Luce

❮ Previous
Next ❯

Citations

Aug 5, 1997·Biotechnology and Bioengineering·Y LeeJ Yin
Dec 1, 1993·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·P Schuster
Sep 1, 1994·Computers & Chemistry·P Schuster, P F Stadler
Jun 30, 1997·Biophysical Chemistry·A Koltermann, U Kettling
Jun 30, 1997·Biophysical Chemistry·J S McCaskill
Jun 5, 2013·Journal of the American Chemical Society·Adrien PadiracYannick Rondelez
Apr 1, 1996·Nature Biotechnology·Y Lee, J Yin
Jun 5, 2003·Chaos·Irving R. Epstein, John A. Pojman
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J S McCaskill, G J Bauer
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jack F DouglasJan Genzer
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tomoaki MatsuuraTetsuya Yomo
Jan 31, 1991·Biochemical and Biophysical Research Communications·J Yin
Jan 7, 2014·Chemistry & Biology·Michael P Robertson, Gerald F Joyce
May 14, 2016·Journal of the American Chemical Society·Ilaria BotteroDouglas Philp
Aug 1, 1995·Analytical Chemistry·P A PiunnoH Cohen
Mar 4, 2018·European Biophysics Journal : EBJ·John S McCaskill
Nov 4, 2017·Chemical Society Reviews·Tamara Kosikova, Douglas Philp
Jun 13, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Vincent FerreiroAlamgir Karim
Jul 20, 2007·Angewandte Chemie·Gerald F Joyce
Apr 9, 2019·Annals of the New York Academy of Sciences·Norikazu Ichihashi
Aug 29, 2002·Journal of the American Chemical Society·Stephanie Thouvenel-RomansOliver Steinbock
Mar 1, 1993·Journal of Bacteriology·J Yin
May 17, 2011·Trends in Ecology & Evolution·Harold P de Vladar, Nicholas H Barton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.