Travelling ultrasound promotes vasculogenesis of three-dimensional-monocultured human umbilical vein endothelial cells.

Biotechnology and Bioengineering
Chikahiro ImashiroKenjiro Takemura

Abstract

To generate three-dimensional tissue in vitro, promoting vasculogenesis in cell aggregates is an important factor. Here, we found that ultrasound promoted vasculogenesis of human umbilical vein endothelial cells (HUVECs). Promotion of HUVEC network formation and lumen formation were observed using our method. In addition to morphological evaluations, protein expression was quantified by western blot assays. As a result, expression of proteins related to vasculogenesis and the response to mechanical stress on cells was enhanced by exposure to ultrasound. Although several previous studies have shown that ultrasound may promote vasculogenesis, the effect of ultrasound was unclear because of unregulated ultrasound, the complex culture environment, or two-dimensional-cultured HUVECs that cannot form a lumen structure. In this study, regulated ultrasound was propagated on three-dimensional-monocultured HUVECs, which clarified the effect of ultrasound on vasculogenesis. We believe this finding may be an innovation in the tissue engineering field.

References

Jun 1, 1991·Stroke; a Journal of Cerebral Circulation·S M Teague, M K Sharma
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·A J ManiotisD E Ingber
Nov 20, 2002·Biochemical and Biophysical Research Communications·Craig B ClarkJohn A Frangos
Apr 20, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mir H AliPaul T Schumacker
Oct 9, 2004·American Journal of Physiology. Heart and Circulatory Physiology·C Radel, V Rizzo
Dec 23, 2004·American Journal of Physiology. Cell Physiology·Akitoshi SasamotoMasahiro Sokabe
Jan 28, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tatsuya ShimizuTeruo Okano
Apr 11, 2008·Tissue Engineering. Part a·Du Geon MoonJames J Yoo
Dec 17, 2008·The Journal of Cell Biology·Margaret L GardelClare M Waterman
Feb 7, 2009·Tissue Engineering. Part B, Reviews·Benjamin D Elder, Kyriacos A Athanasiou
Oct 13, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yu-Ru V ShihOscar K Lee
Mar 9, 2012·Journal of Cell Science·Nathaniel G dela PazPatricia A D'Amore
Aug 24, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Simon W MooreMichael P Sheetz
Jan 31, 2013·Nature Communications·Hidekazu SekineTeruo Okano
Feb 20, 2013·Scientific Reports·Katsuhisa SakaguchiTeruo Okano
Feb 27, 2013·Lab on a Chip·Sudong KimNoo Li Jeon
Aug 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xiaoyun DingTony Jun Huang
Mar 21, 2015·Nature Reviews. Drug Discovery·Eric W EschDongeun Huh
Apr 18, 2015·Frontiers in Bioengineering and Biotechnology·Mikaël M MartinoAndrea Banfi
Jul 4, 2015·Trends in Cell Biology·Anna HaegerPeter Friedl
Nov 26, 2015·Prostate Cancer and Prostatic Diseases·R van VelthovenK Limani
Mar 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Dekel RosenfeldShulamit Levenberg
Apr 15, 2017·Ultrasound in Medicine & Biology·Lauren ManciaEric Johnsen
May 4, 2017·IEEE Transactions on Bio-medical Engineering·Misa NakaoKenjiro Takemura
Dec 20, 2018·Journal of Cellular Physiology·Thaís Silva PintoWillian F Zambuzzi
Feb 26, 2019·Ultrasound in Medicine & Biology·Misa NakaoKenjiro Takemura
Sep 17, 2019·Science Advances·Mark A Skylar-ScottJennifer A Lewis
Jun 13, 2020·Scientific Reports·Chikahiro ImashiroKenjiro Takemura

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