Treadmill exercise enhances therapeutic potency of transplanted bone mesenchymal stem cells in cerebral ischemic rats via anti-apoptotic effects

BMC Neuroscience
Yixian ZhangNan Liu

Abstract

The transplantation of bone marrow stromal cells (MSCs) has proved to ameliorate ischemic brain injury in animals, but most transplanted MSCs undergo apoptosis in the ischemic penumbra, greatly compromising the therapeutic value of this treatment. Meanwhile, cell apoptosis can be inhibited by post-ischemia exercise which has been demonstrated to improve the expression of related anti-apoptotic proteins. The present study investigated whether treadmill exercise enhances the neuroprotective effects of transplanted MSCs in a rat experimental stroke model. Rats were subjected to 2-h middle cerebral artery occlusion (MCAO). Twenty-four hours after reperfusion, they were assigned randomly to receive no MSCs treatment and no exercise (control group), intravenous transplantation of MSCs and treadmill exercise (MSCs + Ex group), MSCs transplantation only (MSCs group) and treadmill exercise only (Ex group). Neurological assessment, TUNEL staining and western blot were performed. Compared with the MSCs group and Ex group, the MSCs + Ex group reported markedly improved neurological function, significantly decreased apoptotic cells, and increased expressions of survivin and bcl-2 (p < 0.05 or p < 0.01, respectively). Interestingly, the trea...Continue Reading

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Citations

Jun 28, 2017·Pediatric Physical Therapy : the Official Publication of the Section on Pediatrics of the American Physical Therapy Association·Chet T Moritz, Fabrisia Ambrosio
Oct 30, 2016·International Journal of Stroke : Official Journal of the International Stroke Society·Qing WuWenchun Qu
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Apr 2, 2020·Current Stem Cell Research & Therapy·Xiaojie BaiHong Zhang
Nov 27, 2021·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Harmanvir GhumanMichel Modo
Jan 22, 2021·Neurology·Leonardo Roever, Glen C Jickling

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Methods Mentioned

BETA
protein assay
electrophoresis
flow cytometry

Software Mentioned

Image J

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