Treating ischemia via recruitment of antigen-specific T cells

Science Advances
Brian J KweeDavid J Mooney

Abstract

Ischemic diseases are a leading cause of mortality and can result in autoamputation of lower limbs. We explored the hypothesis that implantation of an antigen-releasing scaffold, in animals previously vaccinated with the same antigen, can concentrate TH2 T cells and enhance vascularization of ischemic tissue. This approach may be clinically relevant, as all persons receiving childhood vaccines recommended by the Centers for Disease Control and Prevention have vaccines that contain aluminum, a TH2 adjuvant. To test the hypothesis, mice with hindlimb ischemia, previously vaccinated with ovalbumin (OVA) and aluminum, received OVA-releasing scaffolds. Vaccinated mice receiving OVA-releasing scaffolds locally concentrated antigen-specific TH2 T cells in the surrounding ischemic tissue. This resulted in local angiogenesis, increased perfusion in ischemic limbs, and reduced necrosis and enhanced regenerating myofibers in the muscle. These findings support the premise that antigen depots may provide a treatment for ischemic diseases in patients previously vaccinated with aluminum-containing adjuvants.

References

May 4, 1999·The Journal of Clinical Investigation·J M Isner, T Asahara
Oct 25, 2001·Lancet·K Ouriel
Jul 9, 2002·Journal of Molecular and Cellular Cardiology·Dimitri ScholzWolfgang Schaper
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Matthias HeilWolfgang Schaper
Jun 25, 2003·Circulation·Eugenio StabileShmuel Fuchs
Nov 9, 2004·Trends in Immunology·Alberto MantovaniMassimo Locati
Dec 25, 2004·The International Journal of Biochemistry & Cell Biology·Ilaria PuxedduFrancesca Levi-Schaffer
Sep 15, 2005·The Journal of Allergy and Clinical Immunology·Ilaria PuxedduFrancesca Levi-Schaffer
Jan 19, 2007·Journal of Thrombosis and Haemostasis : JTH·E A Silva, D J Mooney
Mar 17, 2007·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Ruth R ChenDavid J Mooney
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanfen LiFabio Re
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kewal AsosinghSerpil C Erzurum
Aug 25, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·V van WeelP H A Quax
Sep 18, 2007·Advanced Drug Delivery Reviews·Lan Cao, David J Mooney
Sep 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eduardo A SilvaDavid J Mooney
May 15, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M L ConradH Garn
Oct 7, 2009·Allergy·I PuxedduF Levi-Schaffer
Mar 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jason D RohChristopher K Breuer
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·William W YuenDavid J Mooney
Oct 6, 2011·Journal of Pediatric Gastroenterology and Nutrition·Eitan RubinsteinDavid H Broide
Jun 14, 2012·The Journal of Biological Chemistry·Ke-Jie YinY Eugene Chen
May 15, 2013·Nature Reviews. Cardiology·Brian H Annex
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anthony O AwojooduEdward Botchwey
Mar 5, 2014·Biomaterials·Kara L SpillerGordana Vunjak-Novakovic
Oct 16, 2014·Annals of Biomedical Engineering·Brian J Kwee, David J Mooney
Oct 22, 2014·Annals of Biomedical Engineering·Kara L SpillerGordana Vunjak-Novakovic
Jan 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Christine A CezarDavid J Mooney
Apr 12, 2017·Diabetes·Martin A ThelinOmar A Ali
Jun 20, 2018·Biomaterials·Brian J KweeDavid J Mooney
Oct 3, 2018·Proceedings of the National Academy of Sciences of the United States of America·Theresa M Raimondo, David J Mooney

❮ Previous
Next ❯

Citations

Jan 24, 2020·Pathogens and Disease·Sonal GuptaRakesh Bhatnagar
Jul 18, 2020·Frontiers in Immunology·Mengjuan Zhang, Song Zhang
Oct 8, 2020·Cell Transplantation·M Rezaa MohammadiJonathan Rt Lakey
Apr 9, 2021·British Journal of Cancer·Anette L Magnussen, Ian G Mills
Jun 1, 2021·Chemical Engineering Journal·Zain SiddiquiVivek A Kumar
Aug 5, 2020·Advanced Drug Delivery Reviews·Alexander J Najibi, David J Mooney
Aug 2, 2021·Molecular Aspects of Medicine·Sabrina L Macias, Benjamin G Keselowsky
Aug 14, 2020·Accounts of Chemical Research·Kwasi Adu-Berchie, David J Mooney
Oct 7, 2021·Science Translational Medicine·Bo Ri SeoDavid J Mooney

❮ Previous
Next ❯

Methods Mentioned

BETA
amputation
fluorescence-activated cell sorting
bronchoalveolar
lavage
enzyme-linked immunosorbent assay
Protein Assay
flow cytometry

Software Mentioned

Immunospot ScAnalysis
ImageJ
GraphPad Prism
LDPIwin

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.