Treatment of acute myeloid leukemia cells in vitro with a monoclonal antibody recognizing a myeloid differentiation antigen allows normal progenitor cells to be expressed

The Journal of Clinical Investigation
Irwin D BernsteinW Raskind

Abstract

Monoclonal antibody L4F3 reacts with most acute myeloid leukemia (AML) cells and virtually all normal granulocyte/monocyte colony-forming cells (CFU-GM). Our objective was to determine whether lysis of AML cells with L4F3 and complement allowed expression of normal myeloid progenitors. The five glucose-6-phosphate dehydrogenase (G6PD) heterozygous patients with AML studied manifested only a single G6PD type in blast cells and in most or all granulocyte colony-forming cells, indicating that the leukemias developed clonally. The cells remaining after L4F3 treatment from two of the patients gave rise to granulocytic colonies that expressed the G6PD type not seen in the leukemic clone, indicating that they were derived from normal progenitors (CFU-GM). L4F3-treated cells from these two patients cultured over an irradiated adherent cell layer from normal long-term marrow cultures also gave rise to CFU-GM, which were shown by G6PD analysis to be predominantly nonleukemic. In the other three patients, the progenitor cells remaining after L4F3 treatment were derived mainly from the leukemic clone. The data suggest that in vitro cytolytic treatment with L4F3 of cells from certain patients with AML can enable normal, presumably highly im...Continue Reading

Citations

Dec 1, 1994·Cancer Immunology, Immunotherapy : CII·K J McGrawD A Scheinberg
Oct 1, 1991·Current Opinion in Immunology·D A Scheinberg
Jul 20, 2001·Hybridoma·K N SyrigosK J Harrington
Oct 24, 2001·Current Opinion in Oncology·E L Sievers, M Linenberger
Jan 31, 2012·Blood·R B WalterIrwin D Bernstein
Jul 5, 2001·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·A E FrankelJ G Jurcic
Jun 12, 2013·Frontiers in Bioscience (Landmark Edition)·Andrew J CowanR B Walter
Feb 28, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Leslie MortlandJatinder K Lamba
Jun 1, 1997·Leukemia & Lymphoma·P Lefterova, I G Schmidt-Wolf
Apr 24, 2002·Clinical Lymphoma·Irwin D Bernstein
Apr 10, 1999·Annals of Diagnostic Pathology·P G ChuL M Weiss
Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L CamposD Guyotat
Oct 26, 2001·Hematology/oncology Clinics of North America·J P Perentesis, E L Sievers
May 18, 2001·Best Practice & Research. Clinical Haematology·G F TorelliR Foà
Jul 21, 2004·Cancer Genetics and Cytogenetics·Lin ChenHervé Tilly
Jul 1, 1995·British Journal of Haematology·I G Schmidt-WolfS Serke
Jun 14, 2017·Leukemia·C D GodwinR B Walter
Jan 13, 2001·Cancer Biotherapy & Radiopharmaceuticals·A E FrankelD A Scheinberg
Jan 1, 1991·Annals of the New York Academy of Sciences·A NajmanM Guigon
Sep 30, 2020·Expert Opinion on Biological Therapy·Benjamin H GoldensonE D Ball
Sep 28, 2001·Hematological Oncology·M Macheta, J A Yin
Jan 1, 1990·Leukemia & Lymphoma·J W Singer, P J Fialkow
Oct 13, 2017·Blood·Frederick R Appelbaum, Irwin D Bernstein

Related Concepts

LIF protein, human
Monoclonal Antibodies
Colony-Forming Units Assay
Glucosephosphate Dehydrogenase
Growth Inhibitors
Genetic Carriers
Alloenzymes
Lymphokines
Colony-forming Unit
Acute Myeloid Leukemia, M1

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